. The microscope; an introduction to microscopic methods and to histology. Microscopes; 1899. 122 MICRO SPECTROSCOPE AND POLAR/SCOPE. [CH. Jtrman Kit Fig. t 15. Various Spectrums —All except that of Sodium were obtained by dif- fused day light with the slit of such a width as gave the most distinct Fraunhofer lines. It frequently occurs that with a substance giving several absorption bands (e. §-., chlorophyll) the density or thickness of the solution must be varied to show all the different bands clearly. Solar Spectrum.— With diffused day-light and a narrow slit the spectrum is not
. The microscope; an introduction to microscopic methods and to histology. Microscopes; 1899. 122 MICRO SPECTROSCOPE AND POLAR/SCOPE. [CH. Jtrman Kit Fig. t 15. Various Spectrums —All except that of Sodium were obtained by dif- fused day light with the slit of such a width as gave the most distinct Fraunhofer lines. It frequently occurs that with a substance giving several absorption bands (e. §-., chlorophyll) the density or thickness of the solution must be varied to show all the different bands clearly. Solar Spectrum.— With diffused day-light and a narrow slit the spectrum is not visible much beyond the fixed line B. In order to extend the visible spectrum in the red to the line A, one should use direct sunlight and a piece of ruby glass in place of the watch glass in Fig. irj. Sodium Spectrum.—The line spectrum (§ 1S2) of sodium obtained by lighting the microscope with an alcohol flame in which some salt of sodium is glowing. With the micro-spectroscope the sodium line seen in the solar spectrum and with the incandescent sodium appears single, except under very favorable circumstances (§ 79i)- By using a comparison spectrum of day-light with the sodium spectrum the light and dark D-lines will be seen to be continuous as here shown. Permanganate of Potash.— This spectrum is characterized by the presence of five absorption bands in the middle of the spectrum and is best shown by using a -^ per cent, solution of permanganate in water in a watch glass as in Fig. T17. Met-hemoglobin.— The absorption spectrum of met-hemoglobin is characterized by a considerable darkening of the blue end of the spectrum and of four absorp- tion bands, one in the red near the line C and two between D and E nearly in the place of the two bands of oxy- hemoglobin ; finally there is a somewhat faint, wide band near F. Such a met-hemoglobin spectrum is best obtained by making a solu- tion of blood in water of such a concentration that the two oxy-hemoglobin bands run
Size: 2370px × 1054px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauth, bookcentury1800, bookdecade1890, booksubjectmicroscopes
