. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY AND BEHAVIOR 221 I. Post-Training Treatment Times Figure 1. Immune/staining intensity levels for calexcitin under riro experimental conditions. First, specimens of Hermissenda were exposed to 10 fig/ml of RGD. a tripeptide. cell adhesion molecule inhibitor, at the designated post-training (nine training events) application times through to 240 min (designated (mint: donhlc-hatched columns). At 240 min. the animals were tested, the RGD removed, and the animals transferred in the training tray to a nih with
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY AND BEHAVIOR 221 I. Post-Training Treatment Times Figure 1. Immune/staining intensity levels for calexcitin under riro experimental conditions. First, specimens of Hermissenda were exposed to 10 fig/ml of RGD. a tripeptide. cell adhesion molecule inhibitor, at the designated post-training (nine training events) application times through to 240 min (designated (mint: donhlc-hatched columns). At 240 min. the animals were tested, the RGD removed, and the animals transferred in the training tray to a nih with running setiwater heing siphoned through each lane until they were tested again at 24-h post-training. Second, replicate sets of animals (solid black columns) were similarly trained but not exposed to RGD or rested, and then decapitated and fixed at time points similar to those for the RGD-exposed animals. An overall control group (single-hatched column) consisted of naive, untrained animals held under similar conditions until they were also decapitated and filed. All central nen'ous svstems were processed collectively for immunocytochemistry. Calexcitin initnunostaining intensity was measured from digital photomicrographs using NIH Image sofnvare. Results were statistica/lv compared using ANOVA and painvise Student's t tests (see text). n = 4-10 eyes measured), whether animals were treated with RGD and tested at 4 and 24 h. or simply trained and fixed at similar time points. The same was true for the second phase (240 min to 24 h post-training). Again, the intensities were statistically identi- cal, whether animals were tested or not. at 240 min or at 24 h (F = ts ; n = 4-10 eyes measured). However. the overall ANOVA between all conditions did show significant differences between the first and second phases (F = : P = <). The principal results obtained from this study indicate that the working hypothesis must be redefined to indicate that, to preserve a me
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
