. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS 361 and taster than video rate imaging devices, such as shuttered cameras, that will maintain the contrast of filaments that are moving, growing or shortening, and also most likely undergoing rapid Brownian motion. For very short exposure times, brighter light sources, such as lasers, may be required as well. I thank Nikon. for their 1992 Nikon Fellowship award. I am indebted to Dr. Shinya Inoue for his generous support and advice and Dr. Lewis Tilney for providing rabbi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS 361 and taster than video rate imaging devices, such as shuttered cameras, that will maintain the contrast of filaments that are moving, growing or shortening, and also most likely undergoing rapid Brownian motion. For very short exposure times, brighter light sources, such as lasers, may be required as well. I thank Nikon. for their 1992 Nikon Fellowship award. I am indebted to Dr. Shinya Inoue for his generous support and advice and Dr. Lewis Tilney for providing rabbit muscle acetone powder. Financial support through research grants R37GM31617-11 from NIH and MCB-8908169 from NSF(both to Dr. Shinya Inoue) and a Swiss National Science Foundation Fellowship is gratefully acknowledged. Literature Cited 1. Yanagida, T., M. Nakase, K. Nishiyama, and F. Oosawa. 198-4. \aiure 307: 58-60. 2. Nagashima, H., and S. Asakura. 1980. J. Mol. Bio/ 136: 169-182. 3. Cassimeris, L., N. K. Fryer, and E. D. Salmon. 1988. J. Cell Biol 107: 2223-2231. 4. Block, S. M., K. A. Fahrner, and H. C. Berg. 1991. J Bacterial. 173: 933-936. 5. Schnapp, B. J., J. Gelles, and M. P. Sheetz. 1988. Cell Motil. Cytoskei 10: 47-53. 6. Inoue, S. 1989. Methods Cell Biol 30: 85-112. Fluorescence Microscopy of Single Actin Filaments Labeled by Conjugation to Rhodamine E. L. Bearer (Dept. of Pathology, Brown University. Providence, Rl. and Marine Biological Laboratory) Actin filaments have traditionally been visualized by electron microscopy or by fluorescence microscopy of filaments saturated with fluorescently labeled phalloidin (1. 2). The conjugation of a rhodamine fluorochrome directly onto actin monomers has made possible the observation of actin dynamics inside living cells (3). These filaments are easily observed when acting as a group or bundle, but are not bright enough to be detected as individual filaments (4). Individual actin filaments directlv con- jugated to fluoresce
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