. Practical text-book of plant physiology. Plant physiology. 258 RESPIRATION, FERMENTATION AND DIGESTION ing the seeds has been calculated the exact amount of the gases interchanged may be found. The Bonnier and Mangin apparatus may be used instead of the one referred to above. 327. Estimation of Atmospheric Gases with Bonnier and Mangin Apparatus. Fill a test-tube with mercury and support mouth downward in a small receiver full of mercury (Fig. 127, /). Con- nect a short bent tube with the outlet of the receiver containing the germinating seeds. Run water into the receiver displacing the air,


. Practical text-book of plant physiology. Plant physiology. 258 RESPIRATION, FERMENTATION AND DIGESTION ing the seeds has been calculated the exact amount of the gases interchanged may be found. The Bonnier and Mangin apparatus may be used instead of the one referred to above. 327. Estimation of Atmospheric Gases with Bonnier and Mangin Apparatus. Fill a test-tube with mercury and support mouth downward in a small receiver full of mercury (Fig. 127, /). Con- nect a short bent tube with the outlet of the receiver containing the germinating seeds. Run water into the receiver displacing the air, and as soon as that in the outlet tubes has been thrown out run the tip under the rim of the inverted test-tube and allow a few cc. to escape into it. Close the receiver and transfer the test-tube to. Fig. 127. Apparatus for determination of atmospheric gases designed by Bonnier and Mangin. a, wheel with handle attached to a piston with threads on its surface: the inner end of the piston projects into the cavity of a cylinder b, which is filled with mercury; revolutions of the piston cause movements out of the cylinder into the graduated glass tube fg, and vice versa, c, metallic fitting, connecting graduated tube with cylinder, d, bulb in glass tube, f, portion of glass tube, calibrated, g, U-shaped portion of glass tube the outer end of which lies underneath the surface of the mercury in the vessel h. m, showing method of placing test-tube containing gas to allow the gas to be drawn into the graduated tube, n, showing the portion of the graduated tube which should be filled by the gas in a test. /, small receiver suitable for containing the solution used in absorbing gases. A similar form is suitable for mer- cury over which the test-tubes may be filled with gas from the bell-jar containing the plant. After Belzung the bowl of the measuring apparatus, holding the thumb over the mouth of the inverted tube enclosing a layer of mercury and the gas to be tested. Press the test-t


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