. The Journal of tropical medicine and hygiene . bdomen doughy onpalpation. No malarial parasites were present inthe peripheral blood, but an increase of large mono-nuclear leucocytes and lymphocyt-es was not^ was present throughout the illness. showing no polar staining. Young cultures on agaralso showed filamentous forms. Biological Characters.—Grew best aerobically at37° C, and was capable of growth at 22° C. and40° C, and was also a facultative anaerobe. Cultural Characters.—On agar thegrowth resembledthat of B. typhosus. In broth a general turbiditywas produced without a pel
. The Journal of tropical medicine and hygiene . bdomen doughy onpalpation. No malarial parasites were present inthe peripheral blood, but an increase of large mono-nuclear leucocytes and lymphocyt-es was not^ was present throughout the illness. showing no polar staining. Young cultures on agaralso showed filamentous forms. Biological Characters.—Grew best aerobically at37° C, and was capable of growth at 22° C. and40° C, and was also a facultative anaerobe. Cultural Characters.—On agar thegrowth resembledthat of B. typhosus. In broth a general turbiditywas produced without a pellicle. Gelatine was notliquefied. Litmus milk was rapidly acidified with-out the formation of a clot. On the eighth day thereaction was changed to a permanent potato a moist, white, viscid growth was organism reduced nitrates to nitrites, but didnot produce indol, and failed to give a positiveVoges Proskauer reaction. Biochemical Reactions.—Acid but no gas wasproduced in glucose, laevulose, galactose, Blood culture was carried out on the eighth dayof the disease. The day following the patientdeveloped a lobar pneumonia with blood-tingedexpectoration. Blood culture yielded a Gram-negative motilebacillus 16 microns long and 0-5 micron broad lactose, raffinose, salicin, glycerol, and mannitol,while neither acid nor gas occurred in rhamnose,saccharose, dextrin, inulin, starch, erythrol, adonitol,dulcitol, or sorbitol. Serum, —The patients serum taken atthe end of the third .week of the illness agglutinated Dec. 2, 1918.] THE JOURNAL OF TROPICAL MEDICINE AND HYGIENE. 231 this bacillus up to a dilution of 1:60, but failed toagglutinate B. typhosus in dilutions of 1:40 and1:60. Specific typhoid serum with a titre of 1 :2000 to1:8000 completely agglutinated this bacillus in adilution of 1 :4000, but no agglutination occurred inhigher dilutions. Specific paratyphoid A and B sera failed toagglutinate this bacillus. Case 3.—An Eg
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