. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 272 FRANK M. FISHER. JR. AND ROBERT O. McALISTER mg protein PH A A 5 - • — • -. protein o —o = activity. p-D 7 -A-A- l_l \ V 400 200 Cl mEq I 500 n n DEAE fraction # FIGURE 5. Purification of trehalase on DEAE cellulose column. Details of elution are presented in the text. l',nenj\ of activation The energy of activation of trehalose hydrolysis was determined from the linear portion (0° to 60°) of an Arrhenius plot (log K vs 1/T) where the slope is equivalent to - (Fig. 6) and found to be 9321 Cal/mole from du
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 272 FRANK M. FISHER. JR. AND ROBERT O. McALISTER mg protein PH A A 5 - • — • -. protein o —o = activity. p-D 7 -A-A- l_l \ V 400 200 Cl mEq I 500 n n DEAE fraction # FIGURE 5. Purification of trehalase on DEAE cellulose column. Details of elution are presented in the text. l',nenj\ of activation The energy of activation of trehalose hydrolysis was determined from the linear portion (0° to 60°) of an Arrhenius plot (log K vs 1/T) where the slope is equivalent to - (Fig. 6) and found to be 9321 Cal/mole from duplicate experiments. It was also observed that 86% of the enzymatic activity remained after one hour at 70° providing substrate was present, whereas 40% of the livdrolytic activity was lost in 10 minutes at 70° in the absence of trehalose. DISCUSSION During the past ten years considerable interest has been directed toward the study of trehalases from divers sources. A resume of these investigations is pre- sented in Table IV. With the identification of trehalose as the predominant cir- culating sugar in insects (Wyatt and Kalf, 1957) many investigations have been directed toward the trehalases from this class of arthropods. The further identi- fication of trehalose in a number of invertebrates with representatives from 12 phyla ( Fairbairn. 1958) and in a number of protists (Cochrane. 1961; Sussman,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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