Archives of internal medicine . ith 90 per cent, alcohol for three to four hours at a temperature of65 to 70 C. The alcohol extract was filtered off (using the centrifuge whennecessary) and evaporated in vacuo over sulphuric acid to dryness. The driedresidue was next extracted with absolute alcohol and the extract filtered. Thisfiltrate was likewise evaporated to dryness in vacuo over sulphuric acid. Theresidue thus obtained was extracted with water, the water solution made alkalinewith sodium carbonate and extracted with ether. The ether solution was allowedto evaporate spontaneously. As this
Archives of internal medicine . ith 90 per cent, alcohol for three to four hours at a temperature of65 to 70 C. The alcohol extract was filtered off (using the centrifuge whennecessary) and evaporated in vacuo over sulphuric acid to dryness. The driedresidue was next extracted with absolute alcohol and the extract filtered. Thisfiltrate was likewise evaporated to dryness in vacuo over sulphuric acid. Theresidue thus obtained was extracted with water, the water solution made alkalinewith sodium carbonate and extracted with ether. The ether solution was allowedto evaporate spontaneously. As this is essentially the Stas-Otto method for theextraction of ptomains, the residue from the ether extract should contain thetoxic substances originally present. Other residues and fractions were alsotested, however, and in those cases in which toxic products were found it wasin fractions other than the last residue described. Table 17.—Average Acidity and Moisture Context of Corn Meal from Various Sources Aciditv, N/10 XaOH. Corn meal from state institutions (8 samples) Corn meal from local grocery store (3 samples) Slightly spoiled meal from Kankakee (1 sample) (not used as food for patients) Good meal from Alabama (3 samples) Damaged meal from Italy (6 samples) Damaged meal from Alabama (3 samples) Very badly spoiled and moldy meal (3 samples) Of the five molds (three varieties of Penicillium, one Mucor and oneMonoscus purpureas), only one, Monascus purpureas, gave a toxic sub-stance when grown on corn meal in pure form. Another sample of meal on which a blue green species of Penicilliumhad grown, but which had become contaminated by other organisms, wasvery toxic. Some further experiments to investigate the presence of toxins incorn were carried out by Captains Xichols and Siler. After taking some corn meal mush from a boiler just before it wasserved and letting it stand in a sterile Petri dish for two days, we foundit covered with slimy, reddish growth, which proved to
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