. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 432 LYNN M. RIDDIFORD DAYS FOR SECOND HOST TO INITIATE DEVELOPMENT (o) 10 20 5-. 0 10 20 DAYS FOR HOST TO INITIATE 30 40 DEVELOPMENT (• 70 FIGURE 1. The average score of the implanted testes, ovaries, and fat body as a function of the mean time (±1 day) required for the initiation of adult development of the host pupa after the implantation. Implants from: closed squares represent untreated fresh « 12-hour old) pupae (15 implants per point until day 8; 6 for day 12; and 3 thereafter) ; closed circles, fresh pupae given 25 /*


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 432 LYNN M. RIDDIFORD DAYS FOR SECOND HOST TO INITIATE DEVELOPMENT (o) 10 20 5-. 0 10 20 DAYS FOR HOST TO INITIATE 30 40 DEVELOPMENT (• 70 FIGURE 1. The average score of the implanted testes, ovaries, and fat body as a function of the mean time (±1 day) required for the initiation of adult development of the host pupa after the implantation. Implants from: closed squares represent untreated fresh « 12-hour old) pupae (15 implants per point until day 8; 6 for day 12; and 3 thereafter) ; closed circles, fresh pupae given 25 /*g C18-JH at onset of preptipal period (at least 15 implants per point except 9 for days 0-3 and 4 for days 3-6) ; triangles, fresh pupae given JH mimics ( 150 Mg JHA, 50 ZR512, or 50 fj-g EGS) at onset of prepupal period (at least 25 implants per point through 15 days, then 12 for days 17-21, and 4 thereafter) ; inverted triangles, fresh pupae given 50 fig EGS as white prepupae (at least 10 implants per point); open circles, isolated abdomens 14-36 days after removal from fresh pupae treated with 25 C18-JH at onset of prepupal period (6 implants per point except only 1 at 14 days). instance. 30 /^g EGS was the minimum dose which prevented diapause, yet treated individuals showed an external score of as well as complete inhibition of metamorphosis of the viscera. Similarly, 10 /Ag ZR512 usually prevented diapause with nearly complete inhibition of internal metamorphosis, but it also partially inhibited the pupal-adult transformation of the integument ( score). Assessment of the developmental status of viscera from JH-treated pupae Thus, a low dose of the animal's own juvenile hormone applied at the onset of the prepupal period interfered with metamorphosis of the viscera without affect- ing the integument. But, as with the previous study with JH-A (Riddiford, 1972'), it was possible that the JH was persisting in the animal through the pre- pupal period and a


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology