. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2 34567 8 9 * Figure 5. Specificity of the polyclonal antiserum raised against OHSS. (A) SDS-PAGE of fractions eluted from molecular-sieve chromatography. The polyacrylamide gel was transblotted onto a PVDF membrane and stained with Coomassie brilliant blue. (B) Immunostaining of the PVDF membrane with a polyclonal antiserum raised to OHSS. Numbers to the left show the molecular masses of the standards shown in Figure 2B. The intensely immunostained bands in fractions 6-8 have masses of about 32 kDa and 30 kDa (arrows to the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2 34567 8 9 * Figure 5. Specificity of the polyclonal antiserum raised against OHSS. (A) SDS-PAGE of fractions eluted from molecular-sieve chromatography. The polyacrylamide gel was transblotted onto a PVDF membrane and stained with Coomassie brilliant blue. (B) Immunostaining of the PVDF membrane with a polyclonal antiserum raised to OHSS. Numbers to the left show the molecular masses of the standards shown in Figure 2B. The intensely immunostained bands in fractions 6-8 have masses of about 32 kDa and 30 kDa (arrows to the right). In this experiment (Fig. 6A), however, the 32-kDa band was at most 2 mm apart from the 30 kDa band on the gel. In addition, the PVDF membrane was not stained with CBB, except the narrow strip that had been cut on both sides (5 mm in width), because the antibodies were not bound to CBB-stained proteins. So these protein bands could have been incompletely separated. In Figure 6B, concentrated active fractions from molec- ular sieve chromatography (6 and 7 in Fig. 2A) were elec- trophoresed for a longer period (9 h), which produced a gap of 5-6 mm between the upper and lower bands. The whole membrane was stained by Ponceau S, and each protein band (2 mm in width) was subjected to affinity purification. Again, the antibody raised from the 32-kDa band bound to the 30-kDa band as well; and the antibody produced from the 30-kDa band also recognized the 32-kDa band (Fig. 6B). kDa. B kDa i a. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983.
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
