. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 114 M. has been produced by the concentrated solution employed. Even with solutions of penetrating substances isosmotic with blood, the swelling that precedes hemolysis is usually indicated by a slight fall in the curve. By using greater dilutions of blood so that the readings appear higher on the scale these effects can be considerably magnified and used to. FIG. 3. Typical record of the partial hemolysis of ox blood in NaCl. The marks on the curve indicate 30 second intervals. good advantage in studying vol
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 114 M. has been produced by the concentrated solution employed. Even with solutions of penetrating substances isosmotic with blood, the swelling that precedes hemolysis is usually indicated by a slight fall in the curve. By using greater dilutions of blood so that the readings appear higher on the scale these effects can be considerably magnified and used to. FIG. 3. Typical record of the partial hemolysis of ox blood in NaCl. The marks on the curve indicate 30 second intervals. good advantage in studying volume changes rather than hemolysis. As would be expected, swollen corpuscles produce lower and shrunken ones higher readings than normal ones, a fact already noted by Holker (1921). In experiments of longer duration, where the method described is wasteful of kymograph paper and fatiguing to the eye of the observer, it is preferable to make readings at regular intervals marked by the writing point itself, allowing the drum to move only enough each time to record the level of the reading. A record of this sort covering minutes with readings every 30 seconds is reproduced in Fig.' 3. It represents the partial hemolysis of ox corpuscles in NaCl slightly buffered for pH with phosphates. When the duration of the experiments is very short, , less than perhaps 10 seconds, kymograph records become difficult to make. Fairly complete and accurate hemolysis curves may be obtained, how- ever, in such cases by setting the instrument in advance at any selected point and determining with a stop-watch the time required to reach this point. The vessel is then emptied and the experiment repeated with a different setting of the instrument, and so on, as many times as desired. The complete curve may then be plotted from the separate points obtained. With experiments of such extremely short duration ( less than. Please note that these images are extracted from scanned page ima
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
