. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. Fig. 2 TABLE IV EFFECT OF SELFDIGESTION OF TRYPSIN ON ITS COMPOSITION Run Incubation time Residual proteolytic activity % Residual protein concentration Ratio r Components I: II from area meas- , , â ^ 0/ (ascending areas) ureinents A I day 54 42 2 B 2 days* 43 34 C 2 days** 89 74 1-7 3 days** 75 71 D 5 days** 63 63 1-9 * Digested in M sodium acetate, M MgCl^, pH ** Digested in M sodium acetate, M CaCl^, pH U
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. Fig. 2 TABLE IV EFFECT OF SELFDIGESTION OF TRYPSIN ON ITS COMPOSITION Run Incubation time Residual proteolytic activity % Residual protein concentration Ratio r Components I: II from area meas- , , â ^ 0/ (ascending areas) ureinents A I day 54 42 2 B 2 days* 43 34 C 2 days** 89 74 1-7 3 days** 75 71 D 5 days** 63 63 1-9 * Digested in M sodium acetate, M MgCl^, pH ** Digested in M sodium acetate, M CaCl^, pH Ultracentrifugation The ultracentrifugal behaviour of trypsin was investigated in several arbitrarily differentiated pH-stability regions. At pH where the enzyme is most stable, sedimenta- tion diagrams characteristic of a homogeneous protein were obtained as is exemplified by the photographs in scries A of Fig. 3. The dependence of the sedimentation constant on the concentration of the enzyme is presented in Table V. No significant concentration dependence of the sedimentation constant below i % protein concentration was observed and the average of the values obtained was s^f^,^ = S. Freshly prepared solutions of trypsin in a borate buffer, pH 8, present similar patterns as shown in scries B of Fig. 3. From the data in Table V it can be seen that the sedimentation constants are not significantly higher at this pH, giving an average value of S2o,a, ^ S. Two runs performed on trypsinogen in the acid and alkaline buffer present identical patterns and sedimentation constants as trypsin. References p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Burk, Dean. Amsterdam, Houston, Elsevier Pub. Co.
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