Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . e prepared with normal blood and txphoid l>()uillon. for purpc^ses oieonijjarison. (See 452, 453. 454.) The time rc(|uired for the completion oi a reaction varies, but in a{general way it may be said that typhoid blood will cause detinite clump-injj and loss of motility of the typhoid bacilli within thirty minutesin the great majority of instances. In some cases a longer time willbe necessary before a correct interpretation of the test can be reaction ttioy not be cl
Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . e prepared with normal blood and txphoid l>()uillon. for purpc^ses oieonijjarison. (See 452, 453. 454.) The time rc(|uired for the completion oi a reaction varies, but in a{general way it may be said that typhoid blood will cause detinite clump-injj and loss of motility of the typhoid bacilli within thirty minutesin the great majority of instances. In some cases a longer time willbe necessary before a correct interpretation of the test can be reaction ttioy not be classed as positive unless both cliinip jormationand loss of motility coexist, and those reactions in lehich either of thephenomena are icantiiii; may not be called typical. For the macroscopic method small test-tubes, the luniina of whichshould not exceed to cm., are used. The examination shouldbe made in series, and for this purpose the tubes may be arranged sideby side. The first tube should contain the bacterial emulsion second tube forty parts of the bacterial emulsion and one part of. I-ic. 454.— Typhmsl^; W iuals Tkst; .V \ few small clumps of bacilli having impaired motility. Persistent motility of the bacteria in other parts of the Ikld occurs. the suspected serum. The third tube receives one jtart of normal serumand forty parts of the liacterial emulsion. Other tubes may be usedcontaining higher dilutions of the suspected serum. In the pres-ence of a positive reaction, the bacteria collect in minute Hocculi (ag-glutination) and finally fall to the bottom of the tube (precipitation).The reactif)n should be complete in from twelve to twenty-four Records.—In order to render comparable studies madeby different investigators, as w^ell as for comparison of the character-istics of different bacteria, it becomes necessary to select some definiteorder of study and a method by which accurate records may be
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