. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NUTRIENT MEDIA AND METHODS OF CULTIVATION. 129 Tig. 62.—Aitken's Tube. and if the contents remain transparent and free from film, they may be set aside as stock-bulbs, to be used when required. To inoculate the liquid in the bulb the end of the neck is heated to sterilise the exterior, the bulb is gently warmed, and the extremity of the neck nipped off with a pair of sterilised forceps. The open extremity is plunged int
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NUTRIENT MEDIA AND METHODS OF CULTIVATION. 129 Tig. 62.—Aitken's Tube. and if the contents remain transparent and free from film, they may be set aside as stock-bulbs, to be used when required. To inoculate the liquid in the bulb the end of the neck is heated to sterilise the exterior, the bulb is gently warmed, and the extremity of the neck nipped off with a pair of sterilised forceps. The open extremity is plunged into the liquid containing the micro-organisms, and a minute quantity enters the tube and mingles with the fluid in the bulb without fear of contamination by atmospheric germs. The extremity of the neck is once more sealed up in the flame of a Bunsen burner. Aitken's Tubes.—These tubes are plugged and sterilised, and the nutrient medium introduced as into ordinary test-tubes. Instead of withdrawing the cotton-wool plug, they are inoculated through a lateral arm. The sealed extremity of the arm is nipped oflf with sterilised forceps, and the inoculating needle is carefully introduced through the opening thus made. It is directed along the arm until it touches the opposite side of the test-tube, where it deposits the material with which it was charged. The needle is withdrawn, and the end of the lateral arm again sealed up in the flame ; the test-tube is then tilted until the liquid touches the deposited material; on restoring the tube to the vertical, the material is washed down with the nutrient liquid. Miguel's Bulbs.—The tube ct boule of Miquel is also a very useful form. It consists of a bulb of 50 cc. capacity, blown in the middle of a glass tube. The part of the tube above the bulb is contracted in two places, and can either be left quite straight or made to curve slightly. Between the contrac- tions the tube is plugged with asbestos. The portion of the tube below the b
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Keywords: ., bookcentury1800, bookdecade1890, bookpublisherphila, bookyear1897
