. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Washed Leukocytes 273 The hands of the patient are washed, and a piece of elastic rubber tubing or some other convenient fillet wound about the thumb or a finger to produce venous congestion. With a convenient lancet (Wright uses a pricker made by drawing a bit of glass tubing or a glass rod to a fine point in the flame) a prick is made about a quarter inch from the root of the nail. From this the blood is permitted to flow into small test-tubes pre-
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Washed Leukocytes 273 The hands of the patient are washed, and a piece of elastic rubber tubing or some other convenient fillet wound about the thumb or a finger to produce venous congestion. With a convenient lancet (Wright uses a pricker made by drawing a bit of glass tubing or a glass rod to a fine point in the flame) a prick is made about a quarter inch from the root of the nail. From this the blood is permitted to flow into small test-tubes pre- viously filled about three-fourths with per cent, sodium citrate solution. The blood and citrate solution are mixed, and the tubes placed in a centrifuge, balanced, and centrifugalized until the corpuscles are collected at the bottom of the tube. The citrated plasma i* now with- drawn and replaced with per cent, sodium chloride solution, through which the corpuscles are distributed by shaking. The tubes are now again centrifugalized until the corpuscles are collected, when the saline is removed carefully, the last drop from the back of the meniscus. In the corpuscular mass that remains the leuko- cytes form a thin creamy layer on the top. The serum to be tested and the normal serum for comparison are secured in the same manner, the former from the patient, the latter from the operator. As it is advisable to wound the patient 'S. Fig. 96.—Tube of blood and citrate solution before and after centrifugaliz- ing (Miller).. Fig. 97.—Removing last drops of saline solution (Miller). but once, the tube for obtaining the serum should be filled at the same time that the citrated blood is taken. The blood to furnish the serum is taken in a small bent tube shown in the illustration. 18. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly r
Size: 1830px × 1365px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
