. Ecological study of the Amoco Cadiz oil spill : report of the NOAA-CNEXO Joint Scientific Commission . Figure 2. Locations of sampling sites. aerobic techniques designed for cultivation of bacteria with extreme sensitivity to oxygen (Hungate, 1969). Cores were sectioned into de- sired intervals and subcores removed by a No. 4 cork-^borer or by a 3 ml syringe with the end of the barrel cut off ( mm ). The 0-3 cm in- terval was used for all the oil and mousse addition experiments. For these experiments an anoxic slurry was made by mixing the core section with 20% (V/V) anoxic artifical sea
. Ecological study of the Amoco Cadiz oil spill : report of the NOAA-CNEXO Joint Scientific Commission . Figure 2. Locations of sampling sites. aerobic techniques designed for cultivation of bacteria with extreme sensitivity to oxygen (Hungate, 1969). Cores were sectioned into de- sired intervals and subcores removed by a No. 4 cork-^borer or by a 3 ml syringe with the end of the barrel cut off ( mm ). The 0-3 cm in- terval was used for all the oil and mousse addition experiments. For these experiments an anoxic slurry was made by mixing the core section with 20% (V/V) anoxic artifical seawater (ASW, Burkholder, 1963). In experiments on hydrocarbon metabolism slurries of other depth intervals were made in the same way. Subsamples ( ml) from core sections or slurries were transferred to 2 dram glass vials (Acme Vial and Glass Co.) and sealed under a stream of helium. The helium was passed over heated copper filings to remove any traces of oxygen. Vials were sealed with 00 butyl rubber stoppers ( Thomas). Unless noted be- low, all isotope additions ( ml) were taken from sterile anoxic stock solutions with a 1 ml helium flushed glass syringe (Glaspak). Mousse, oil and hydrocarbon additions were added to vials containing sediment under a flow of helium gas using a 1 ml pipet 12 hours before microbial activities were assayed. Benzene and toluene were added with a 5 pi syringe (Hamilton). Measurement of Microbial Activities All incubations were done at ambient temperatures (20-24°C). Com- parisons between different samples were made by a two sample t test, using the AN0V1 program of MSUSTAT (Lund, 1979). 162
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