. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. CI (blue) CI (red) which on reduction becomes the following colorless compound CI HO /~\ NH /~^\ OH It will be seen that the type of color change shown by this in- dicator is very similar to that of litmus (see p. 217). Litmus is a non-synthetic product of unknown chemical composition; it is not believed to be related to the indophenols, in spite of its similar be- havior as an acid-base and oxidation-reduction indicator. There i


. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. CI (blue) CI (red) which on reduction becomes the following colorless compound CI HO /~\ NH /~^\ OH It will be seen that the type of color change shown by this in- dicator is very similar to that of litmus (see p. 217). Litmus is a non-synthetic product of unknown chemical composition; it is not believed to be related to the indophenols, in spite of its similar be- havior as an acid-base and oxidation-reduction indicator. There is only one other dye of this group that need be mentioned here. t20 indophenol blue c. I. NO .8ii Synonym: Indophenol. CII3 0-. ~ _ _, . N. CH3 CisHieNzO; Mol. Wt. {Absorption maximum in alcohol about 592) This compound is occasionally referred to in biological work. Herxheimer (1901), for example, employed a dye which he called indophenol (and which probably was indophenol blue), in satu- rated solution in 70% alcohol, as a fat stain. It is still sometimes used for the same purpose; see Black (1938), for instance. 3. THE THIAZINS The thiazins constitute one of the most important groups of dyes from the standpoint of the biologist; while for textile dyeing the group contains but a small number of dyes of any importance. In these compounds, as mentioned above, the two benzene rings are further joined by a sulfur atom. Considerable information is at hand concerning the spectro-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications


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