. The Biological bulletin. Biology; Zoology; Marine biology. 178 J. W. SCHNETZER AND M. S. TYLER Mouth hooks Labial disc Clypeo- labnuii disc Esophagus Anlenna- eye disc Brain. Posterior midinlestine Posterior spiracle Figure 2. Diagram of endogenous ^-galactosidase activity in the late third-instar larva stained at pH After X-gal staining, /3-galactosidase activity (black) is evident in the anterior and posterior spiracles, distinct segments of both the anterior and posterior midintestine, cellular epi- dermis, lymph glands, and eye-antenna imaginal discs. (Anatomy after Bodenstein, 1965
. The Biological bulletin. Biology; Zoology; Marine biology. 178 J. W. SCHNETZER AND M. S. TYLER Mouth hooks Labial disc Clypeo- labnuii disc Esophagus Anlenna- eye disc Brain. Posterior midinlestine Posterior spiracle Figure 2. Diagram of endogenous ^-galactosidase activity in the late third-instar larva stained at pH After X-gal staining, /3-galactosidase activity (black) is evident in the anterior and posterior spiracles, distinct segments of both the anterior and posterior midintestine, cellular epi- dermis, lymph glands, and eye-antenna imaginal discs. (Anatomy after Bodenstein, 1965; Tyler, 1994.) In the prepupal stage P4, the amount of foamy-ap- pearing tissue with diffuse stain increased, and stain was now found throughout the developing intestinal system. The fat body showed light staining. Again, there was no staining in the brain or ventral ganglion. Pupal stages (P5-P15). During metamorphosis, the delicate nature of the remodeling tissue disallowed whole- mount dissections, but X-gal staining of cryostat serial sections revealed dramatic changes in staining intensity and distribution (Figs. 6 and 7). After head eversion, P5, and in stage P6, there was an increase in dark, punctate staining which co-localized with the fat-body tissue of the head capsule, thorax, and ab- domen. By stage P6, dark staining was associated with the first pupal spiracles and trachea. The staining asso- ciated with foamy-appearing tissues was no longer found in the head region but persisted in the developing legs Ring gland Corpus allatum R-othoracic gland Trachea Lymph glands Aorta Gastric caeca. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biologic
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