. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AVIAN RELATIONSHIPS 200 RESULTS The following tables indicate some of the results obtained when the native and extracted antigens were tested with precipitating antisera by ring and photronreflectometric tests. The homologous titer indi- cated over each antiserum is in terms of protein rather than nitrogen content. In the body of the table the homologous titers and areas are valued at 100 per cent. The heterologous titers and areas are expressed in per cent values which indicate their relation to the homologous values. A das
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AVIAN RELATIONSHIPS 200 RESULTS The following tables indicate some of the results obtained when the native and extracted antigens were tested with precipitating antisera by ring and photronreflectometric tests. The homologous titer indi- cated over each antiserum is in terms of protein rather than nitrogen content. In the body of the table the homologous titers and areas are valued at 100 per cent. The heterologous titers and areas are expressed in per cent values which indicate their relation to the homologous values. A dash indicates that no test was made. Figure 1 is a graphic representation of the total area of reaction between a diluted (1+2) (one part serum plus two parts of buffered sa- line) anti-leghorn serum and each of several antigens. While the even- numbered tube numbers have been omitted from the figure to prevent over-crowding, the turbidity values obtained with the even-numbered tubes will be found on the graph. In all figures, tube number 1 is a dilution of one part of protein to parts of saline; tube number 2 is a dilution of one part of protein to of saline; tube number 3 a dilution of one part to 125; each succeeding tube possessing one-half of the concentration of protein of its predecessor. In a similar manner the crystalline egg albumins, hemoglobins, and lens-proteins were used to determine relationships. Six antisera made to the hemoglobins of leghorn, duck, and turkey could not distinguish between the heterologous and homologous antigens by ring test. A similar condition was experienced with five antisera made to the lens proteins of leghorn, duck, and turkey. With the photronreflectometer, however, the same antigens and antisera reacted to give values com- parable to those obtained with the serum-proteins. The following table shows some of the values indicated by ring and photronreflecto- metric tests. DISCUSSION The results tabulated here represent a portion
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
