. The British journal of dermatology . culture histologically showed the typicalmycelium and spores of sporothrix—mycelium slender, about 2 diameter, septated and branching, and spores, pear-shaped or oat-shaped, 3-5 mm. in length, attached to and arranged round themycelium, in some places so closely together as to hide the myceliumand form strings or cylindrical masses of about 10 mm. in diameter. 272 A CASK OF SPOROTRICHOSIS. Tliere were many scattered spores detached in tlie process ofpreparation. Prof. J. Alfred Scott kindl\- examined my cultures and microscopicpreparations and confi
. The British journal of dermatology . culture histologically showed the typicalmycelium and spores of sporothrix—mycelium slender, about 2 diameter, septated and branching, and spores, pear-shaped or oat-shaped, 3-5 mm. in length, attached to and arranged round themycelium, in some places so closely together as to hide the myceliumand form strings or cylindrical masses of about 10 mm. in diameter. 272 A CASK OF SPOROTRICHOSIS. Tliere were many scattered spores detached in tlie process ofpreparation. Prof. J. Alfred Scott kindl\- examined my cultures and microscopicpreparations and confirmed the diagnosis. I found two methods of staining good. One suggested to me byProf. Henry Dixon, Professor of Botany, Trinity College, Dublin^viz. stain the dried and fixed culture witli 50 per cent. DelafielcVslogwood for ten minutes; then wash, dehydrate with alcohol, andmount in Canada balsam*: The other, the formic acid and borax methylene blue method, whichSabourand recommends for staining the hairs and scales of Fig. -2. Technique.—A drop of water is placed in the centre of a slide. Aminute amount of the culture is taken with a sterilised platinumneedle and transferred to the drop of water, and very gently movedabout in the water to dissociate it, if contact with the water does notimmediately lead to dissociation. Eough treatment would separatethe spores from the mycelium. The slide is then placed in an incubator till the water hasevaporated, or it may be allowed to diy in the air. The film is then fixed by placing over it a drop of formic acid, andthen heating the slide till the formic acid steams, letting it cool andheating again ; this process is repeated a few times. The slide is then washed in water. The fixed film is then stained with borax methylene blue (Sahli). HOVAI. SOOIKTY OK iMKDKMNK. 27S When staining hairs of ringworni a Tuinute is usually sufficient. Butin the staining of sporothrix, Mr. Pelli>siei- (who was acting as mjclinical clerk
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Keywords: ., bookcentury1800, bookdecade1880, booksubjectsyphilis, bookyear188
