. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 238 JOHN G. MONER RESULTS A. Quantitative relations of growth and colony production At the outset of this work on Pcdiastrmn it was desirable to obtain large num- bers of swarming colonies in a relatively short time. To accomplish this, large inocula of 5 cc. for every 50 cc. of fresh medium were used in preparing cultures. "When this was done it was noticed that swarming occurred in great numbers at a specific time after inoculation and that it seemed to last for only a few days. The time that swarming or colony produc
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 238 JOHN G. MONER RESULTS A. Quantitative relations of growth and colony production At the outset of this work on Pcdiastrmn it was desirable to obtain large num- bers of swarming colonies in a relatively short time. To accomplish this, large inocula of 5 cc. for every 50 cc. of fresh medium were used in preparing cultures. "When this was done it was noticed that swarming occurred in great numbers at a specific time after inoculation and that it seemed to last for only a few days. The time that swarming or colony production began could be predicted with reasonable accuracy in succeeding cultures inoculated in the same manner from the same stock. Virtually no swarming was found in the cultures prior to this swarming period and after it was completed. CONT. •j U. 100 200 TIME IN HRS. I 2 FIGURE 1. Graph showing colony production and cleavage activity curves. Open circles represent colony production and black dots represent cleavage activity. FIGURE 2. Graph showing colony production in fresh or control medium and in aged medium after 0 (E-l), 1 (E-2) and 2 (E-3) days' growth in fresh or control medium. In order to obtain a quantitative description of colony formation as a function of time the following experiment was performed: a culture was started consisting of 50 cc. of fresh medium inoculated with a 5-cc. suspension of 99-day old stock cul- ture and grown in a 125-cc. flask. The lighting was provided by a 100-watt tung- sten filament bulb and the experiment run at room temperature (24—28° C.; see Materials and Methods). One-cc. aliquots were removed daily from the culture, fixed and colony number determined using a Levy hemacytometer chamber. In the results in Figure 1 it is seen that no colony production occurs in the culture up to 17-i hours but that a sudden burst begins at 197 hours and lasts for 78 hours at. Please note that these images are extracted from scanned page images that
Size: 2006px × 1246px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
