. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 494 R. E. STEPHENS a masking effect since the cilia are at least twice as "hot" as the axonemes, contain twice as much protein, and this protein (chiefly membrane, matrix, and cell debris) is of nearly constant activity. A B C D E F G mm - DYNEIN. FIGURE 3. Acrylamide gel autoradiograms of ciliary axonemes. A—stained gel, 27-30 hour pulse period; B and C—autoradiograms of 6-9 hour and 12-15 hour pulse period gels; D, E, and F—autoradiograms of 24-27 hour, 27-30 hour, and 30-33 hour pulse period gels, respectively;
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 494 R. E. STEPHENS a masking effect since the cilia are at least twice as "hot" as the axonemes, contain twice as much protein, and this protein (chiefly membrane, matrix, and cell debris) is of nearly constant activity. A B C D E F G mm - DYNEIN. FIGURE 3. Acrylamide gel autoradiograms of ciliary axonemes. A—stained gel, 27-30 hour pulse period; B and C—autoradiograms of 6-9 hour and 12-15 hour pulse period gels; D, E, and F—autoradiograms of 24-27 hour, 27-30 hour, and 30-33 hour pulse period gels, respectively; G—autoradiogram of gel from regenerated axonemes from the 27-30 hour pulse period. Slight misalignment of bands is due to uneven drying of gels. Several bands are virtually indetectable in the stained gel, but are apparent in the autoradiogram due to high specific activity resulting from dc noro synthesis (cf. Fairbanks ct al., 1965). All gels were loaded with the same amount of axonemal protein. Over the moderately constant range of ciliary protein incorporation, the nxoneme represents about of the total incorporated radioactivity but accounted for only about of the total embryonic protein by actual measurement after isolation. A very rough calculation of the expected amount of total axonemal protein, based on one 30 /j. cilium per cell per 500-cell blastula in a sample of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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