. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. ELECTRON TRANSPORT IN R. RUBRUM 283. 400 450 500 WAVELENGTH (m^j) 550 600 Fig. 6. Action spectrum of light-stimulated respiration of "young" light-grown cells of R. nibnon whose light-sensitive respiration had been depressed approx. 95 per cent with M '^-butanol. The ratio of dark to light respiration was determined to be In this experiment, the cell suspension was previously mixed with M n-butanol and the assay was carried out under air or a gas mixture of CO : O2 = 4:1. In assay, irradiation by scann
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. ELECTRON TRANSPORT IN R. RUBRUM 283. 400 450 500 WAVELENGTH (m^j) 550 600 Fig. 6. Action spectrum of light-stimulated respiration of "young" light-grown cells of R. nibnon whose light-sensitive respiration had been depressed approx. 95 per cent with M '^-butanol. The ratio of dark to light respiration was determined to be In this experiment, the cell suspension was previously mixed with M n-butanol and the assay was carried out under air or a gas mixture of CO : O2 = 4:1. In assay, irradiation by scanning lights (variable in wavelength) was immediately followed by diphasic response under the gas mix- ture containing CO, but monophasic under air. During the time required for arrangements in this assay, the respiration was altered in response to light so that light stimulation was observed. The action spectrum of the light-stimulated respiration thus altered was measured with some experimental difficulties. In assay under the gas mLxture, the null balance between the scanning and control (550 m/i) lights was made for only the initial phase of response. Using air in- stead of the gas mixture gave similar results, but the'response was monobasic (20). Taylor (unpublished) obtained similar action spectra, using cells which were so aged that their respiration was stimulated by light (see text). action spectra for light-stimulated respiration with aged cells resemble the absorption spectrum of bacteriochlorophyll treated with ferric chloride or iodine (25). It seems likely that oxidation products of bacteriochlorophyll cause the oxygen uptake stimulated by illumination, Geller and Lipmann (26) have reported that some pigments having peaks between 420 m/z and 435 mfi are reduced when the chromato-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfec
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