. Bulletin. Plant diseases -- United States. leaves, a dendritic yeast, and a nondendritic yeast (both yeasts were obtained from the sticky surface of Niagara grapes). In all of these cases the gas soon foamed over the top of the test tube. An old coconut culture of Px. hyacinth! also gave a very considerable quan- tity of gas. The least amount of gas was obtained from adding HgO^ to 3-months- old potato cultures of Jones' carrot rot bacillus. Three tubes were tried, all of which behaved alike. At first there was no gas, then a slow, long continued evolution of small bubbles, the total not l)e
. Bulletin. Plant diseases -- United States. leaves, a dendritic yeast, and a nondendritic yeast (both yeasts were obtained from the sticky surface of Niagara grapes). In all of these cases the gas soon foamed over the top of the test tube. An old coconut culture of Px. hyacinth! also gave a very considerable quan- tity of gas. The least amount of gas was obtained from adding HgO^ to 3-months- old potato cultures of Jones' carrot rot bacillus. Three tubes were tried, all of which behaved alike. At first there was no gas, then a slow, long continued evolution of small bubbles, the total not l)eing very great. A young potato culture of this bacillus (8 days old) yielded gas almost from the start and in much greater quantity than the old cultures. The reverse was true of Ps. campestris. A potato culture ?> months old yielded gas more promptly and in greater volume than did a vigorous culture made from the same tube and only S days old. Both, how- ever, yielded nuich gas. On the con- trary, even that from the young cultures of Jones's bacillus fell far behind in amount that which was evolved by the other 10 bacteria and by the two yeasts. Some gas was also obtained by pouring H3O2 upon old rice cultures of various funo'i, e. 2"., Fusarmm niveuri). F. vasin- feet aril, Swingle's Atta fungus (culti- vated by the writer from a nest of Atta, near Washington), and from an agar plate culture of cotton anthracnose. The yield of gas from the fungi named was insigniticant in comparison with that obtained from the yeasts and from the bacteria, exclusive of the old cultures of Jones's bacillus. The other bacilli and the two 3^easts gave so much gas that the tubes were tilled and frothed over, usually within a few minutes. In the accompanying illustration (fig. 2) a 3-months-old potato culture of Ph. pkmeoli to which H.,0., has been added is shown by the side of a check tube (uninoculated) to which H^Oj has also been added. In the one case there was a very copious evolution
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