. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 264 L. E. DEATON 16 14 12 10 8 6-- 2- 1 0 Cont 5HT FMRF pQD FLRF DMF 4|3 PDA PMA Figure 6. The effects of various agents on the release ot'amino acids from Mercenaria ventricles transferred to isosmotic seawater (1000 mOsm). Each bar represents the mean of live ventricles; error bars are 1 SD. Treatments: Cont = controls; 5HT = 5-hydroxytryptamine (10~6 A/); FMRF = FMRFamide (10 " A/); pQD = pQDFLRFamide (10'" M): FLRF = FLRFamide (10"" .M): DMF = dimethylformamide ( ml/); 4/3 = 4-beta-phorbol (10~
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 264 L. E. DEATON 16 14 12 10 8 6-- 2- 1 0 Cont 5HT FMRF pQD FLRF DMF 4|3 PDA PMA Figure 6. The effects of various agents on the release ot'amino acids from Mercenaria ventricles transferred to isosmotic seawater (1000 mOsm). Each bar represents the mean of live ventricles; error bars are 1 SD. Treatments: Cont = controls; 5HT = 5-hydroxytryptamine (10~6 A/); FMRF = FMRFamide (10 " A/); pQD = pQDFLRFamide (10'" M): FLRF = FLRFamide (10"" .M): DMF = dimethylformamide ( ml/); 4/3 = 4-beta-phorbol (10~7 A/): PDA = phorbol (10~7A/):PMA = phorbol 12-acetate, 13-myristate (10 7 A/). The wet weights of ventricles incubated in 500 mOsm SW containing 10 6 M 5HT are lower than control tis- sues at 10, 30, and 60 min (t = ; P< ; t = , P < ; t = , P < ; respectively) (Figs. 7a, b). Ventricles incubated in 500 mOsm SW containing forskolin (10 5 M) gain significantly more weight than controls in the first 10 min (t = P < ), but there is no difference in wet weight gain 30 and 60 min after transfer (Figs. 7a, b). Phorbol 12-acetate. 13-myristate (10~7 M) reduces the changes in weight relative to control tissues at 10, 30, and 60 min (t = , P < ; t = P < ; t = , P < , respectively) following transfer from 1000 mOsm SW to 500 mOsm SW (Fig. 7c). Tissues trans- ferred from 1000 mOsm SW to 500 mOsm SW contain- ing phorbol show significantly higher (t = P < ) weight gain than control tissues 10 min after transfer, and significantly lower (t = , P < ) weight gain 30 min after transfer. There is no significant difference between these tissues and controls 60 min after transfer (Figs. 7a. c). The changes in wet weight of ventricles transferred from 1000 mOsm SW to isosmotic SW containing FMRFamide. 5HT. and phorbol esters are shown in Fig- ure 8. There
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