. Laboratory work in bacteriology. Bacteriology. 516 BACTERIOLOGY. The contents of each of the capillary tube's are then in- oculated into bouillon which is set aside at 37° to allow the growth, it any, to develop. The contents are removed as follows: The tube is first wiped dry and then one end is slightly scratched with a file. The end is then removed and the opening of the tube is sterilized by touching it 2 or 3 times to a flame. The tube is held in a broad-pointed pair of forceps, and, when cool, the cut end is inserted into the culture tube while the closed end is slowly brought 'jnto co
. Laboratory work in bacteriology. Bacteriology. 516 BACTERIOLOGY. The contents of each of the capillary tube's are then in- oculated into bouillon which is set aside at 37° to allow the growth, it any, to develop. The contents are removed as follows: The tube is first wiped dry and then one end is slightly scratched with a file. The end is then removed and the opening of the tube is sterilized by touching it 2 or 3 times to a flame. The tube is held in a broad-pointed pair of forceps, and, when cool, the cut end is inserted into the culture tube while the closed end is slowly brought 'jnto contact with a flame. The vapor thus pro- duced promptly expels the liquid from the capil- lary. The student should make suspensions and test one or more of the following' or- ganisms, according to the directions given above, Chol- era vibrio, Typhoid bacillus. Anthrax < bacillus (homogeneous cultv/re), Anthrax spores (p. 291) and Streptococcus pyogenes. Action of moist heat at 58°.—The capillary tubes should be with- drawn at the. end of 5, 10, 15, 30 and 60 minutes' exposure. Action of moist heat at 70°.—The capillary tubes are withdrawn at the end of 1, 3, 5 and 10 minutes. In addition to this the anthrax spore tubes should be exposed for 15, 30 and 60 Fig. 75. Determination of tlie tiiermal death-point {F, G. N.)- Action of moist heat at 100°.—The capillary tubes are withdrawn at the end of 1, 2 and 3 minutes. Additional anthrax spore tubes are ex- posed for 5, 10 and 15 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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