. Laboratory work in bacteriology. Bacteriology. 268 BACTERIOLOGY. The hair is removed from the neck by means of a pair of bent scissors. The part should then be shaven clean and washed with an antiseptic. A median incision is made along the neck with a sterile scalpel, and the skin is loosened from the subcutis by using the handle of the knife, or the fingers. The external jugular vein which lies on the side, covered by subcutaneous tissue, is now exposed as carefully as possible. The size of the vein is such that no difflculty will be ex- perienced in finding or entering it. A ligature shoul
. Laboratory work in bacteriology. Bacteriology. 268 BACTERIOLOGY. The hair is removed from the neck by means of a pair of bent scissors. The part should then be shaven clean and washed with an antiseptic. A median incision is made along the neck with a sterile scalpel, and the skin is loosened from the subcutis by using the handle of the knife, or the fingers. The external jugular vein which lies on the side, covered by subcutaneous tissue, is now exposed as carefully as possible. The size of the vein is such that no difflculty will be ex- perienced in finding or entering it. A ligature should then be ap- plied to the vein and the wound in the skin, sewed up as neatly as possible. A few tufts of cotton should be spread over the incision, and then moistened with coUodium. When this has dried the animal can be Fig. 4S. Latapie's animal holder. In the above method of inoculation, every possible pre- caution must be taken to' prevent accidental infection. The hands, as well as the neck of the animal, must be thoroughly disinfected. All instruments, ligatures, etc., must be sterile. The instrument sterilizing case, shown in Fig. 48, p. 275, is used for this purpose. Intravenous injection is practised frequently during the immunization of horses against diphtheria, pest, etc. The operation as made on the horse is relatively as easy as the injection into the veins of the ear of a rabbit. An at- tendant should compress the jugular vein with the thumb, while the operator cuts away the hair over the vein. This cut area is then thoroughly rubbed with lysol, or some other disinfectant. The skin is first pierced with a lance, and then the trochaf is inserted as nearly parallel to the vein as possible. After withdrawal of the trochar, the opening should be compressed by a finger, and eventually it may be coated with Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and ap
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