. Chemical embryology. Embryology. SECT. 9] PROTEIN METABOLISM 1071 icoag. protein nitrogen (o) \ per 100 gma. luncoag. protein niCrogen(»)J wet egg white. Oay= -^- Fig. 306. Vladimirov S(,Schmidtt nitrogen in the proteins not so coagulable plus that of all other nitro- genous compounds, on the other. Owing to protein combustion, the former diminishes and the latter rises. Idzumi, on the contrary, obtained the non-protein nitrogen separately from the uncoagulable protein nitrogen, and the free amino nitrogen in addition to that. The free amino nitrogen remains almost constant during develop- m
. Chemical embryology. Embryology. SECT. 9] PROTEIN METABOLISM 1071 icoag. protein nitrogen (o) \ per 100 gma. luncoag. protein niCrogen(»)J wet egg white. Oay= -^- Fig. 306. Vladimirov S(,Schmidtt nitrogen in the proteins not so coagulable plus that of all other nitro- genous compounds, on the other. Owing to protein combustion, the former diminishes and the latter rises. Idzumi, on the contrary, obtained the non-protein nitrogen separately from the uncoagulable protein nitrogen, and the free amino nitrogen in addition to that. The free amino nitrogen remains almost constant during develop- ment, thus agreeing with the data already presented, but the total non-protein nitrogen rises owing to the accumulation of protein waste products. The protein just referred to, which is not coagulable with acetic acid, is, of course, ovomucoid, and in the Section on carbohydrate meta- bolism an account has been given of our knowledge of the physiology of this compound. Trichloracetic acid was found by Hiller & van Slyke to make a sharp separation between proteins of all kinds and nitrogenous bodies of lower molecular weight. Sakuragi gave for zero hour of de- velopment the values of 1846 mgm. per cent, of coagulable protein nitro- gen (acetic acid) and 209 mgm. per cent, not so coagulable. Using tri- chloracetic acid, Needham found i960 mgm. per cent, for all protein nitrogen and 90 mgm. per cent, non-protein nitrogen. The increase in the protein nitrogen caught by the trichloracetic acid method over that caught by the simple boiling with acetic acid was thus 114 mgm. per cent. Komori found by alcohol precipitation 480 mgm. of ovomucoid per egg, 124 mgm. per cent, of ovomucoid nitrogen, which gives close agreement with the extra 114 found on using trichloracetic acid as the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not pe
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