. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. METAMORPHOSIS IN HA Ml.\.IHA 139. 100-1 Figure 1. Isolation of metamorphic indueer with high performance liquid chromatography (HPLC). Data are the results of partitioning the indueer from a crude methanol extract with a buffer gradient from ab- solute methanol to absolute water (A) and partitioning the partially pu- nned active fractions from A with an isocratic buffer system (absolute methanol) (B). In both A and B, the chromatograph indicates absorbance of each fraction aseluted from an RP-C18 column, detected at 254 nm,
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. METAMORPHOSIS IN HA Ml.\.IHA 139. 100-1 Figure 1. Isolation of metamorphic indueer with high performance liquid chromatography (HPLC). Data are the results of partitioning the indueer from a crude methanol extract with a buffer gradient from ab- solute methanol to absolute water (A) and partitioning the partially pu- nned active fractions from A with an isocratic buffer system (absolute methanol) (B). In both A and B, the chromatograph indicates absorbance of each fraction aseluted from an RP-C18 column, detected at 254 nm, and the histogram gives results of the bioassay used to test the inductive potential of each fraction. In A, bioassay results are the percentage (mean ± standard error) of Haminaea callidegenita veligers that underwent intracapsular metamorphosis in response to seawater (SW), with untreated indueer (EMJ), with a crude EMJ extract (all in hatched bars), as well as with the indicated HPLC fractions (solid bars) pooled over 2-min intervals throughout a 30-min elution period. Peaks of activity are cross- referenced to the chromatograph. In B, results include the percentage of intracapsular metamorphosis occurring in response to standard controls (hatched bars) as well as in response to the fractions indicated in the chromatograph (solid bars), n = number of egg masses assayed. Isolation of the metamorphic indueer has been exten- sively studied in two species of opisthobranch, but in both cases the indueer has yet to be identified. Veligers of the nudibranch Pht'stilla silwgae are induced to metamor- phose by a water-borne compound that is released from its prey, the hard coral Porites (Hadfield, 1977). This compound is small (200-500 Da) and stable to temper- ature and pH (Hadfield and Scheuer, 1985). The nudi- branch Eitbranchus doriae also metamorphoses in re- sponse to its prey species, the hydroid Kirchenpaiicria. Please note that these images are extracted from scanned page i
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
