. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ACTION OF IONIZING RADIATIONS. IV 47 performed with sperm suspensions so diluted. The sperm suspensions from 20 ir- radiated vials were collected in an Erlenmeyer flask from which 3 cc. were pipetted to each Warburg vessel. Thus, every experiment consisted of six control vessels and six vessels with irradiated sperm. Because of the reports in the literature on the resistance of respiration to x-ray irradiation, it was decided to start with 20,000 r. Such irradiation produced a marked inhibition of respiration, which in- crea
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ACTION OF IONIZING RADIATIONS. IV 47 performed with sperm suspensions so diluted. The sperm suspensions from 20 ir- radiated vials were collected in an Erlenmeyer flask from which 3 cc. were pipetted to each Warburg vessel. Thus, every experiment consisted of six control vessels and six vessels with irradiated sperm. Because of the reports in the literature on the resistance of respiration to x-ray irradiation, it was decided to start with 20,000 r. Such irradiation produced a marked inhibition of respiration, which in- creased in the second hour (Fig. 1). The degree of inhibition varied from sample to sample in all experiments. As an example of this variation the data of experi- ments of irradiation with 20,000 r are given in Table II. From this x-ray dose the amount of irradiation was diminished to 100 r with which an inhibition of 10 per cent was observed (Table III). The O2 uptake inhibition on irradiation with 100 r did not increase in the second hour after irradiation (Fig. 2). In previous work 60 40 2 20 o o CJ O. 30 60 90 TIME IN MINUTES 120 FIGURE 2. The effect of x-ray irradiation on the respiration of sea urchin sperm. Irradiation, 100 r. 1. Control; 2. Irradiated Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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