. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MKTAI'. IX SEA URCHIN KMI'.KYOS 449. UP C B MB G PR PL KM,i KK 5. Incorporation ot' <;! \ctTol-l-C14 into the total lipid of sea urchin t-mhryos during the early development. Abbreviations as in Figure 1. methanol 4:1) contained much radioactivity. When a .sample- of this peak was chromatographed on silicic acid-impregnated paper, the spot of phosphatidylethanol- amine was obtained. The third peak (elttted by chloroform-methanol 1:4) was the largest, but contained much less radioactivity. Paper chromatography
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MKTAI'. IX SEA URCHIN KMI'.KYOS 449. UP C B MB G PR PL KM,i KK 5. Incorporation ot' <;! \ctTol-l-C14 into the total lipid of sea urchin t-mhryos during the early development. Abbreviations as in Figure 1. methanol 4:1) contained much radioactivity. When a .sample- of this peak was chromatographed on silicic acid-impregnated paper, the spot of phosphatidylethanol- amine was obtained. The third peak (elttted by chloroform-methanol 1:4) was the largest, but contained much less radioactivity. Paper chromatography showed that this peak contained lecithin. The second and the fourth peaks, which appeared to be inositnl phospholipids and lysolecithin. respectively, were the minor components, and not much radioactivity was located in them. Thus, it appeared very clearly that the cephalin fraction was labeled with acetate-1-C" much more strongly than the lecithin and other fractions. The results obtained with several developmental stages are summari/.ed in Table II. The same samples as those in Figure 3 were fractionated through silicic acid columns. Since, (.specially with lipids obtained from the embryos at later de- velopmental .stages, fraction 1 was sometimes followed by the elution of triglycerides and it was impossible to separate fraction 1 from fraction 2. these two tractions were combined in this table. < ML the whole, no great change was observed in the distribution pattern of C" among lipid fractions during embryonic development, the increase in the labeling of almost all fractions being parallel to the rise in the incorporation into the total lipids. In this experiment, too. fraction 7 was most strongly labeled with acetate-1-C14 throughout development, as indicated by both the total and the -pecitic activities. The total incorporation into the triglyceride fraction was again found to be high; the specific activity was. however, rather Please note that these im
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
