. Biological structure and function; proceedings. Biochemistry; Cytology. SOLUBILIZATION' AND PROPERTIES OF THE DPXH DEHYDROGENASE II3 Within the pH range where assays based on l\^^^ vakies are rehable (5 -5 to 7 • 8) no definite optimum is attained, but at fixed ferricvanide concentra- tions (i-66 X io~^ M or lower) the apparent optimum is around pH 8 (Fig. 10). 0-2- With DPNH With dithionite. 400 550 450 500 Fig. II. Absorption spectrum of dehydrogenase in soluble extract, prior to purification and the effects of DPXH and of dithionite on the spectrum. Protein concentration, 3-6 mg. per ml.;
. Biological structure and function; proceedings. Biochemistry; Cytology. SOLUBILIZATION' AND PROPERTIES OF THE DPXH DEHYDROGENASE II3 Within the pH range where assays based on l\^^^ vakies are rehable (5 -5 to 7 • 8) no definite optimum is attained, but at fixed ferricvanide concentra- tions (i-66 X io~^ M or lower) the apparent optimum is around pH 8 (Fig. 10). 0-2- With DPNH With dithionite. 400 550 450 500 Fig. II. Absorption spectrum of dehydrogenase in soluble extract, prior to purification and the effects of DPXH and of dithionite on the spectrum. Protein concentration, 3-6 mg. per ml.; pH = 7-15. Recorded with Cary Model 11 spectrophotometer. Kinetics of bleaching (at 410) 0 -0-02 -004 DPNH T - L_- 0 30 60 (sec) Fig. 12. Kinetics of bleaching by 6-5 x 10^^ m DPXH. Conditions as Fig. II. Certain characteristic features of the absorption spectrum are readily recognizable in the initial soluble extract, prior to purification (Fig. ii). The main peak (upper curve, oxidized enzyme), which at pH 7-4 is located at 410 nijn, is not a Soret band but is strongly reminiscent of that. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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