. The Biological bulletin. Biology; Zoology; Marine biology. ACID-BASE STATUS IN OYSTERS 143 o S o o a: 20 PcOj 80 PcOj 40 16- 12- Crassostrea virginica. Highly Infected PCO22 PH Figure 2. A pH-HCOj" diagram showing the acid-base status of oyster hemolymph at 0, 5, and 24 h of air exposure at 30°C. PCO2 isopleths (curved lines) are given in torr. In vitro buffer lines are shown as dashed lines. Circles represent oysters uninfected with Perkinsus marinus: triangles represent oysters with "high" infections. Values are mean ± SEM; n for each experiment ranged fr
. The Biological bulletin. Biology; Zoology; Marine biology. ACID-BASE STATUS IN OYSTERS 143 o S o o a: 20 PcOj 80 PcOj 40 16- 12- Crassostrea virginica. Highly Infected PCO22 PH Figure 2. A pH-HCOj" diagram showing the acid-base status of oyster hemolymph at 0, 5, and 24 h of air exposure at 30°C. PCO2 isopleths (curved lines) are given in torr. In vitro buffer lines are shown as dashed lines. Circles represent oysters uninfected with Perkinsus marinus: triangles represent oysters with "high" infections. Values are mean ± SEM; n for each experiment ranged from 22 to 56. At 30°C, however, the slopes of the lines were significantly different (P = ; df = 5). Infection levels Mean P. marinus infection levels of oysters collected in South Carolina in 1993 peaked in September (x = ± ). The mean level was ± in July 1993 and ± in November 1993 (Fig. 3). Throughout the study period, bioassays for the detection of P. marinus were performed on more than 200 South Carolina oysters. Only two oysters were found to be infection-free; thus the infection prevalence was 99%. Among all treatments, the infection level for the control Maryland oysters was zero. In fact, throughout the study, in no case was infection detected in the Maryland oysters (n = 159). Discussion The use of oysters from two different populations (Maryland and South Carolina), while not ideal, was nec- essary because no uninfected oysters were available from South Carolina. Nevertheless, we feel that the physiolog- ical effects we observed in this study were induced by infections of P. marinus and not by differences between the oysters from different sources. Most of the results pre- sented here were obtained from uninfected (Maryland) oysters and highly infected (South Carolina) oysters. South Carolina oysters with infection intensities intermediate between these two extremes (, oysters with "low" in- fections) show intermediate r
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