. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. Fig. 59.—Slide with Gold-le.\f Electrodes. The liquid containing the micro-organisms is placed between the electrodes, covered with a cover-glass, and then subjected to the electric current. (g) Methods of Employixg and Stoking Liquid Media. Cultivations in liquid media can be carried on in test-tubes, but it is more satisfactory to employ special forms of flasks, bulbs, and (J tubes, such as those employed by Pasteur a
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. Fig. 59.—Slide with Gold-le.\f Electrodes. The liquid containing the micro-organisms is placed between the electrodes, covered with a cover-glass, and then subjected to the electric current. (g) Methods of Employixg and Stoking Liquid Media. Cultivations in liquid media can be carried on in test-tubes, but it is more satisfactory to employ special forms of flasks, bulbs, and (J tubes, such as those employed by Pasteur and his school, and by Lister, Sternberg, and Aitken. Lister s Flasks.—These flasks were especially introduced by Lister as a means of storing liquid nutrient media. They are so constructed that after removal of a portion of the contents, on restoring the vessel to the vertical position, a drop of liquid always remains in the extremity of the nozzle, which jjrevents I'egurgitation of unfiltered air. Sternberg's Bulbs.—The method of intro- ducing liquid into the bulb employed by Sternberg, and of .stei-ilising and inoculating it, is as follows : The bulb is heated slightly over the flame, and the extremity of the neck, after the sealed point has been broken off, is plunged beneath the surface of the liquid. As the air cools the liquid is drawn into the bulb, usually filling it to about one-third of its capacity. The neck of the flask is again sealed up, and the. Fig. 60.—Lister's Flask. Fig. 61.—Stekn- bekg's Bdlb. hquid which lias been introduced is sterilised by repeatedly boiling the flasks in the water-bath. They should then be placed in the incubator for two or three days ;. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Crookshank, Edgar M. (Edgar March), 1858-1928. Philadelphia, W. B. Saunder
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Keywords: ., bookcentury1800, bookdecade1890, bookpublisherphila, bookyear1897
