. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 410 LEONARD MUSCATIXK AX!) HOWARD M. LENIIOI-T The culture medium ("M" solution) for this species of hydra consisted of 10~3 M CaCL, 10 :i AI NaHCO3. 10 ' M MgCL, 10 4 M KC1, and 10'3 M tris-(hydroxy- methyl)-aminomethane buffer (Sigma "121"), pH , in water deionized by pass- ing distilled water through organic removal and ion exchange resin columns (Barnstead Black and Red Cap Cartridges). The experimental rationale for the selection of this medium is given in the Results section (Fig. 2). Penicillin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 410 LEONARD MUSCATIXK AX!) HOWARD M. LENIIOI-T The culture medium ("M" solution) for this species of hydra consisted of 10~3 M CaCL, 10 :i AI NaHCO3. 10 ' M MgCL, 10 4 M KC1, and 10'3 M tris-(hydroxy- methyl)-aminomethane buffer (Sigma "121"), pH , in water deionized by pass- ing distilled water through organic removal and ion exchange resin columns (Barnstead Black and Red Cap Cartridges). The experimental rationale for the selection of this medium is given in the Results section (Fig. 2). Penicillin G (sodium, , 50 ) was occasionally added to stock cultures to retard growth of contaminating microorganisms, hut was omitted from cultures during experiments. 4000 £ 2000 00 1000 K = 234567 DAYS FIGTKK 1. (iiouth curvt s tor a >tock culture of ('. 7v'nWm/w<; (preen) obtained by counting (upper curve) or individual hydra (lower curve). Sampling period during logarith- mic growth indicated by bracket. Aposymhiotic control animals (referred to hereafter as albinos) were obtained by growing green individuals for eight days in culture solution containing M glycerine (Whitney, 1907, 1908). Following this treatment, the absence of algae was confirmed by microscopic examination of macerated tissues and of 2- micron sections of paraffin-embedded whole animals. Aposymbiosis was perma- nent. Occasionally, algae reappeared in some individuals soon after glycerine treatment, apparently as a result of incomplete removal of algae. Under con- ditions defined elsewhere in this paper, albinos grew normally when returned to glycerine-l'ree culture solution, and have continued to do so through three years. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woo
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