Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . the hair shaft. The myceUum is found without, but never within thehairs (Hyde). These fungi may be stained by the method of Morris and Calhoun. Thehair is first washed in ether to remove all fatty debris; it is then put for one 1 Triestley (Ann. Trop. Med. and Parasitol., 1914, VIII, 113) reports the presence of themicrosporon scorteum in a case of ringworm. PARASITES. 167 or two minutes in Grams iodin solution and is stained after drying for fromone to five minutes in gentian-violet.
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . the hair shaft. The myceUum is found without, but never within thehairs (Hyde). These fungi may be stained by the method of Morris and Calhoun. Thehair is first washed in ether to remove all fatty debris; it is then put for one 1 Triestley (Ann. Trop. Med. and Parasitol., 1914, VIII, 113) reports the presence of themicrosporon scorteum in a case of ringworm. PARASITES. 167 or two minutes in Grams iodin solution and is stained after drying for fromone to five minutes in gentian-violet. It is again dried and treated for a minuteor two with the iodin solution and for an equal length of time in aniline oilcontaining pure iodin, after which it is cleared with aniline oil, washed in xylol,and mounted in Canada balsam, (3). Microsporon audouini (Trichophyton Microsporon). This parasite appears under the microscope chiefly in the form of a largenumber of round spores, irregularly grouped or massed about the follicularportions of the hair. Mycelial threads, large and branching, are often seen. Fig. 64.—Achorion schonleinii, X 500 diameters. (Van Harlingeji.) within the hair. The sheath of spores surrounding the hair is often con-tinued upward for 1/16 to 1/8 inch above its exit from the follicle and maybe recognized as a whitish or grayish coating of the hair. These mycelialthreads are all within the hair proper, thus differing from those of the tricho-phyton which are never within the hair; after repeatedly dividing and subdivid-ing they terminate on the outer surface of the shaft in fine filaments, at theextremities of which are the spores. This parasite is the cause of the diseasetinea tonsurans, or ringworm of the scalp. (4). Microsporon parasite is readily recognized by the microscopic examination of thescales scraped from the skin. Innumerable clustered spores, highly refractiveand resembling in their circular and oval contours droplets of oil, ar
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