. Ciba Foundation Symposium on Transplantation. Transplantation of organs, tissues, etc. 82 A. A. KANDUTSCH AND JACK H. STIMPFLING. Fig. 5. Effects of some enzymes on the turbidity of a TSL suspension. In experiment A, TSL was sus- pended in 0-iM-tris-maleate buffer pH 7-3. In experiment B, the buffer was o-iM-tris, pH 7-9, except that o-iM-phosphate, pH 6-6, was used when digestion was with pepsin. The concen- tration of TSL was approximately 0-25 Concentrations of enzymes were: trypsin (crystal- hzed), o-i ; a-chymotrypsin (from ethanol), o-i ; pepsin (crystallized), 0-
. Ciba Foundation Symposium on Transplantation. Transplantation of organs, tissues, etc. 82 A. A. KANDUTSCH AND JACK H. STIMPFLING. Fig. 5. Effects of some enzymes on the turbidity of a TSL suspension. In experiment A, TSL was sus- pended in 0-iM-tris-maleate buffer pH 7-3. In experiment B, the buffer was o-iM-tris, pH 7-9, except that o-iM-phosphate, pH 6-6, was used when digestion was with pepsin. The concen- tration of TSL was approximately 0-25 Concentrations of enzymes were: trypsin (crystal- hzed), o-i ; a-chymotrypsin (from ethanol), o-i ; pepsin (crystallized), 0-2 ; Hpase from porcine pancreas (Cal- Biochem., B grade), 0-2 unit/ml.; and lyophilized snake {Crotalus adamanteus) venom from Ross Allen Reptile Institute, o-o8 the presence of a major component and a small amount of a second component (Fig. 6). In phosphate buffer pH 6*3, only one component (mobility 4-06 x io~^ cm.^ ;^ volt~^) was evident. The mobilities of both components at pH 8 • 6 were considerably. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on Transplantation (1961 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); Cameron, Margaret P. Boston, Little, Brown
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