. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2 ABRAMOWITZ, HISAW AND PAPANDREA were always separated into individual containers and starved for at least 5 days previously. 1. THE EFFECT OF A SINGLE INJECTION OF EYESTALK EXTRACT Twenty-one crabs were prepared as previously described. The average blood sugar value for this group was 20 mgs. per cent. The animals were divided into four groups of five animals each, so chosen that the average value of each group was 20 ± 1-2 mgs. per cent. Into each animal, cc. of a sea-water soluble frac- tion of the eyestalks of Uca p
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2 ABRAMOWITZ, HISAW AND PAPANDREA were always separated into individual containers and starved for at least 5 days previously. 1. THE EFFECT OF A SINGLE INJECTION OF EYESTALK EXTRACT Twenty-one crabs were prepared as previously described. The average blood sugar value for this group was 20 mgs. per cent. The animals were divided into four groups of five animals each, so chosen that the average value of each group was 20 ± 1-2 mgs. per cent. Into each animal, cc. of a sea-water soluble frac- tion of the eyestalks of Uca pugilator was injected, this volume being equivalent to one Uca eyestalk. Blood samples were taken from the first group one hour fol- lowing injection, from the second group 2 hours after injection of this group, from the third at 3 hours after injection, from the fourth 4 hours after injection, and 100 o> u 0> E o o» 3 OT TJ O C± tO. Hours after injection FIGURE 1. Curve showing the effect of a single injection of eyestalk extract (Uca) on the resting blood sugar level of the blue crab. again from the first group 5 hours after the injection of this group. The results of this experiment are shown in Figure 1. The diabetogenic effect of the eyestalk extract is not due to reducing substances contained in the extract for, in the above experiment, the amount of extract injected into each animal contained only mgs. per cent reducing substances calculated as glucose. 2. THE EFFECT OF DILUTION ON THE DIABETOGENIC ACTION OF EYESTALK EXTRACTS Eyestalks of Uca pugilator were ground thoroughly with a small amount of sea- water, the soluble portion decanted, and the residue re-extracted twice in the same fashion. The combined soluble portions were centrifuged, and from the super-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
