. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EQUILIBRIUM OF OXYGEN WITH HEMOCYANIN 251 DISCUSSION In the forty years since Hiifner suggested the application of the mass law to the equilibrium between oxygen and hemoglobin, numerous investigations have indicated that equations similar to those employed in the present treatment are more or less adequate to describe the data in hemoglobin solutions free of electrolytes (Barcroft, 192cS). Un- certainty has sometimes accompanied the results of such investigations because of the instability of purified hemoglobin solutions (
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EQUILIBRIUM OF OXYGEN WITH HEMOCYANIN 251 DISCUSSION In the forty years since Hiifner suggested the application of the mass law to the equilibrium between oxygen and hemoglobin, numerous investigations have indicated that equations similar to those employed in the present treatment are more or less adequate to describe the data in hemoglobin solutions free of electrolytes (Barcroft, 192cS). Un- certainty has sometimes accompanied the results of such investigations because of the instability of purified hemoglobin solutions (Ferry, 1924; Hecht, Morgan and Forbes cited by Barcroft, 1928). In the presence of electrolytes and in blood, the dissociation curves of hemoglobin in- variably have a sigmoid shape, requiring some additional assumptions for their explanation. /o y. ,y»H B3/ K-G3SS + -pn 3 71 K-OSB6 ID<se f<-o. 170 IB FIG. 3. Data of oxygen dissociation curves of hemocyanin of Liuiiihis polyphemus plotted to show the similarity of shape at various pH values, y is fraction of hemocyanin present as oxyhemocyanin; p is oxygen pressure in ram. Hg; temperature, 25° C. The curve corresponds to equation (9) when K = \, »= 1. In the case of hemocyanin solutions, Stedman and Stedman (1928) found that the respiratory pigment of the snail, poinatla. com- bines oxygen in accordance with the mass law, as expressed in equation (8), n being taken to be The hemocyanin of the Crustacea, Houi- arus I'ulgaris and Cancer pac/unis, according to these investigators (1926 a, 1926 b) is characterized by oxygen dissociation curves of a more complex nature when examined in dialyzed solution. The present in- vestigation of Linntliis hemocyanin indicates that this substance, when in " salt free " solutions, resembles the hemocyanin of Hcliv in its con- formity to the mass law. Stedman and Stedman, in discussing their observations on 17. Please note that these images are extracte
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