The microscope; an introduction to microscopic methods and to histology . At the ordinary temperature of a sittingroom (20 degrees centigrade) the connective tissue will be so far gelatinized infrom one to three days that it is easy to separate the fascicles and fibers either withneedles or by shaking in a test tube or reagent vial (Fig. 143) with water. Ittakes longer for some muscles to dissociate than others, even at the same temper-ture, so one must try occasionally to see if the action is sufficient. When it is, theacid is poured off and the muscles washed gently with water to remove the
The microscope; an introduction to microscopic methods and to histology . At the ordinary temperature of a sittingroom (20 degrees centigrade) the connective tissue will be so far gelatinized infrom one to three days that it is easy to separate the fascicles and fibers either withneedles or by shaking in a test tube or reagent vial (Fig. 143) with water. Ittakes longer for some muscles to dissociate than others, even at the same temper-ture, so one must try occasionally to see if the action is sufficient. When it is, theacid is poured off and the muscles washed gently with water to remove the one is ready to make the preparations at once they may be isolated and mountedin water. If it is desired to keep the specimen indefinitely or several days, thewater should be poured off and a half saturated solution of alum added ($ 314).The alum solution is also advantageous if the specimens are to be stained. The 176 COLLODION SECTIONING [CH. VII specimens may be mounted in glycerin, glycerin jelly or balsam. Glycerin jellyis the most satisfactory, Fig. 146. Pfeiffers preparation microscope with erecting prism between theobjective and ocular {Leiiz ; Wm. Krafft, New York). THE PREPARATION OF SECTIONS OF TISSUES AND ORGANS $ 265. At the present time there are three principal methods of obtainingthin sections of tissues and organs for microscopic study. These methods are :The Collodion Method, the Paraffin Method, and the Freezing Method. Each ofthese methods has its special application, although the collodion method is per-haps the most generally applicable, and the freezing method the most restricted,and is used mostly in pathological work where rapid diagnosis is necessary andthe finest details of structure are not so important. With the paraffin method thethinnest sections may be made, and in some ways it is the most satisfactory of good microtome is of great aid in sectioning. \ 266. The Collodion Method.—In sectioning by this method the tissues a
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectmicrosc, bookyear1901
