. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 404 IKEGAMI, KANATANI AND KOIDE. FIGURE 1. Sperm-shedding of an isolated gonadal fragment of Leptosynapta inJuicrcns treated with 1-methyladenine: a shows a gonadal fragment before treatment with 1-methyl- adenine; />. the same fragment, contracting and shedding sperm immediately after the treatment with 1 methyladenine at the concentration of 5 X 10~" M. Scale bars equal 1 mm. purine, 7-methyladenine, 7-methylguanine, 6-dimethylaminopurine and 1-methyl- adenosine, failed to induce sperm-shedding at the concentration
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 404 IKEGAMI, KANATANI AND KOIDE. FIGURE 1. Sperm-shedding of an isolated gonadal fragment of Leptosynapta inJuicrcns treated with 1-methyladenine: a shows a gonadal fragment before treatment with 1-methyl- adenine; />. the same fragment, contracting and shedding sperm immediately after the treatment with 1 methyladenine at the concentration of 5 X 10~" M. Scale bars equal 1 mm. purine, 7-methyladenine, 7-methylguanine, 6-dimethylaminopurine and 1-methyl- adenosine, failed to induce sperm-shedding at the concentration of 5 X 10" M. It should 1)6 pointed out that both 1-methyladenine and 1-ethyladenine were unable to induce spawning (Fig. 2) or maturation (germinal vesicle breakdown) of immature oocytes in the gonad. However, some gonadal fragments which con- tained mature oocytes whose germinal vesicle had already disintegrated responded immediately to 1-methyladenine (5 X 10" M) by shedding of these mature oocytes from the cut surface. Gonads filled with mature oocytes could be obtained from only two individuals during the period of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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