. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 390 WALTER AUCLAIR AND DOUGLAS MARSLAND Paramecium: comparative observations Generally speaking, the pressure-temperature effects on Paramecium and Blepharisma were similar. However, there were two important differences: 1) Paramecium was distinctly more sensitive to pressure lysis, and 2) decompression lysis, which was almost negligible in Blepharisma, became very significant in Paramecium. For Paramecium, the critical pressure for 50 per cent lysis was 2000-3000 psi. lower than for Blepharisma, at each of the two temperatu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 390 WALTER AUCLAIR AND DOUGLAS MARSLAND Paramecium: comparative observations Generally speaking, the pressure-temperature effects on Paramecium and Blepharisma were similar. However, there were two important differences: 1) Paramecium was distinctly more sensitive to pressure lysis, and 2) decompression lysis, which was almost negligible in Blepharisma, became very significant in Paramecium. For Paramecium, the critical pressure for 50 per cent lysis was 2000-3000 psi. lower than for Blepharisma, at each of the two temperatures (20° and 25° C.) which were studied. Under such critical conditions (, 7000 ° C.) the animals shortened moderately and displayed gradually diminishing, distinctly ir- regular locomotion, which ceased only if and when cytolysis occurred. Most of the cytolysis occurred during the last 5 minutes of the 15-minute compression period. Moreover, two somewhat different types of lysis were observed with roughly equal frequency. One type seemed to involve a detachment of the pellicle, with the formation of one or more large hyaline blisters which later broke, liberating the deeper granular cytoplasm (Fig. 5). The other type, in contrast, seemed to lOOr. 0 FIGURE "3 8 9 10 II PRESSURE- 1000 LBS. / SQ. IN. FIGURE 3. Blepharisma : percentage of cytolysis as a function of pressure, at four temperatures. Cytolyzed cells were counted exactly 15 minutes subsequent to pressure Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Labor
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