. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 342 Tetanus twenty-four hours, during which time all of the contained micro- organisms, including the tetanus bacillus, increased in number. He then exposed it for an hour to a temperature of 8o°C., by which all fully developed bacteria, tetanus as well as the others, and the great majority of the spores, were destroyed. As scarcely anything but the tetanus spores remained alive, their subsequent growth gave a fairly pure culture. Cultivation.—The tetanu
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 342 Tetanus twenty-four hours, during which time all of the contained micro- organisms, including the tetanus bacillus, increased in number. He then exposed it for an hour to a temperature of 8o°C., by which all fully developed bacteria, tetanus as well as the others, and the great majority of the spores, were destroyed. As scarcely anything but the tetanus spores remained alive, their subsequent growth gave a fairly pure culture. Cultivation.—The tetanus bacillus is difficult to cultivate because it will not grow where the smallest amount of free oxygen is present. It is hence a typical obligatory anaerobe. Farran* and Grixoni. rig. 125.—Bacillus tetani; five-day-old colony upon gelatin containing glucose. X 1000 (Frankel and Pfeiffer). believe it to have originally been an optional anaerobe, and it is said by these writers that the organism can gradually be accustomed to oxygen so as to grow in its presence. When this is achieved, it loses its virulence. The general methods for the cultivation of anaerobic organisms, are given under the appropriate heading (Anaerobic Cultures), and need not be repeated here. The colonies of the tetanus bacillus, when grown upon gelatin plates in an atmosphere of hydrogen, resemble those of the well- known hay bacillus. There is a rather dense, opaque central mass surrounded by a more transparent zone, the margins of which con- sist of a fringe of radially projecting bacilli. Liquefaction occurs slowly. * "Centralbl. f. Bakt. u. Parasitenk.," July 15, i8g8, p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original McFarland, Joseph, 1868-. Philadelphia and London, W. B. Saunders Company
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
