. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIFE HISTORY OF P. JUGOSUS 117. Figure 5. Phase/fluorescence micrographs of DAPI-stained amoebae. A,B. Amoeba with metaphase nucleus. Phase micrograph shows nucleus clearly intact and under fluorescence, DNA is seen condensed in the center. C,D. Amoebae with telophase nucleus. D,F. Cytoplasmic DNA is faintly visible in the amoe- bae. E,F. Arrow indicates a structure which may be a chromatin body extruded by the amoeba. Its size and fluorescent properties differ from cytoplasmic fluorescence. Bar scale = 10 ^m. nonspecific br
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIFE HISTORY OF P. JUGOSUS 117. Figure 5. Phase/fluorescence micrographs of DAPI-stained amoebae. A,B. Amoeba with metaphase nucleus. Phase micrograph shows nucleus clearly intact and under fluorescence, DNA is seen condensed in the center. C,D. Amoebae with telophase nucleus. D,F. Cytoplasmic DNA is faintly visible in the amoe- bae. E,F. Arrow indicates a structure which may be a chromatin body extruded by the amoeba. Its size and fluorescent properties differ from cytoplasmic fluorescence. Bar scale = 10 ^m. nonspecific brightly staining material in the preparations. Unstained amoeba slide preparations were also used as controls to detect and distinguish autofluorescence from authentic DNA staining. Excitation filter sets on the Ni- kon Fluophot in the UV and blue (approximately 365 nm and 490 nm) were used for epifluorescence observations of DAPI and mithramycin samples, respectively. Photo- micrographs were made with a Nikon Microflex AFX photomicrographic attachment. Films used for photomi- croscopy were Scotch 640 ASA tungsten film and Kodak Tri-X 400 ASA pushed to 1600. Electron microscopy Fixation and embedding of the Cuba strain of P. jugo- sus. Amoebae inoculated into aqueous suspension (de-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
Size: 1480px × 1687px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
