. Contributions from the Botanical Laboratory, vol. 12. Botany; Botany. 28 STAIN TEC HNOLOGY â ^^v^"*"''*'^'' M. Debchiption of Plate 1 iron Wvifera infecte,' -^^^'^ f^'"^ ^^^j ^ ,,{,«ââ .ith no ^ t orseillin BB and crystal v,olet. ^'g- ^-^^^^J^; . ,,rv, n. a. ; Fig. 2 with a Spencer -r;-^^"te;. Fi^s'ttaTd ':! a^Zei. oi. i.â⢠fluoHte, â⢠a. fi^^Z Hb XC ocnla. .a. used on a,l photomicrographs. C ^. -a in Conant. . .ru U. Host ceâs and hyphae . tlfixed as hollow structures. Magnification. X300. Ml "'1* L. <<'JI I ^j ^ THE
. Contributions from the Botanical Laboratory, vol. 12. Botany; Botany. 28 STAIN TEC HNOLOGY â ^^v^"*"''*'^'' M. Debchiption of Plate 1 iron Wvifera infecte,' -^^^'^ f^'"^ ^^^j ^ ,,{,«ââ .ith no ^ t orseillin BB and crystal v,olet. ^'g- ^-^^^^J^; . ,,rv, n. a. ; Fig. 2 with a Spencer -r;-^^"te;. Fi^s'ttaTd ':! a^Zei. oi. i.â⢠fluoHte, â⢠a. fi^^Z Hb XC ocnla. .a. used on a,l photomicrographs. C ^. -a in Conant. . .ru U. Host ceâs and hyphae . tlfixed as hollow structures. Magnification. X300. Ml "'1* L. <<'JI I ^j ^ THE FIXING AND STAINING OF ROOT TIPS 29 Ri:suLTs usenii, but the addition of other reuL'ents imt>rr.,.«= .f c i- ⢠which increase tl,e J! '^ "*' 'mproves its fixation image, dehvde hnt w inl '"'^'^'''';,''»^ I*"'"" concentration of the formal- dehyde but which apparently do not penetrate the .s,,ecimen ahead of Ts tforteThv " concentration of the fluid. This supported by a comparison between specimens j, in a for maldehyde fixative containing the acid .salt, ferric diloride andlhose irslZrr' '" ' f"'''''- r^'"â¢"^' ''' relative,; w'r â¢stances, phenol, resorcinol, or pyrogallol (Fig. 3). A mixture of ferric Salicylic acid in all fluids tried gave useful images, but when com bined with chromic acid (Cohen, 1934) it was not a satis actorH when combined either with formaldehyde alone or with forma^Sthyd mixed with chromic sulfate in preserving the cell walls and the hyphae of the fungus (Figs. 2 and 6). ^' Tnl\ T!^ '" ' i 'f "'"' ""'^ f°â¢a'dehyde showing the fixation of the cell w,lls of he host tissue and the intimate association of the intracellular hvphae The ul cells on the nght show the hyphae partly digested. Magnification: X3oi. Figure 3. Fixed in ferric chloride and formaldehyde. The heavily colored spherical ismic Figure 4 Fixed in chr
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Keywords: ., bookcentury1900, bookpublisherphiladelphiasn, booksubjectbotany
