. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 288 DAVID A. RINGLE AND PAUL R. GROSS B B. YP YC * WC (DESCENDING) FIGURE 3. Electrophoretic patterns of soluble yolk fractions. Faster A and slower B components present in all fractions tested. Slow trailing peak (not labeled) an artifact and does not represent a yolk component. that this peak was made up of at least two fractions. The stationary peak trailing the B peak in all runs is an artifact and does not represent an electrophoretic component. 4. Phosphorus/nitrogen ratios Phosphorus/nitrogen (P/N) ratios as well as s
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 288 DAVID A. RINGLE AND PAUL R. GROSS B B. YP YC * WC (DESCENDING) FIGURE 3. Electrophoretic patterns of soluble yolk fractions. Faster A and slower B components present in all fractions tested. Slow trailing peak (not labeled) an artifact and does not represent a yolk component. that this peak was made up of at least two fractions. The stationary peak trailing the B peak in all runs is an artifact and does not represent an electrophoretic component. 4. Phosphorus/nitrogen ratios Phosphorus/nitrogen (P/N) ratios as well as some dry weight/nitrogen ratios are given in Table II. Whole washed platelet suspension, YP (with one excep- tion), and WC gave identical P/N ratios of The P/N ratios for a single batch of YC and YP were slightly lower (), and values as low as were obtained for lipid-extracted YP (YPdf). "Ghost" material gave much higher P/N ratios, Aging of yolk platelets and YP under refrigeration (up to 21 days), and dialysis of YP against refrigerated distilled water for 4 days, did not alter yolk P/N ratios. Nitrogen determinations on the total "ghost" and soluble yolk portions of three different batches of washed yolk platelets showed that the "ghost" fraction repre- sented a fairly constant proportion of the total washed preparation (Table III). This finding indicates that the homogenization-centrifugation technique for isolating yolk may be relied on to produce similar yolk preparations from different batches of Rana pipiens ovaries. 5. Nucleic acids Nucleic acid determinations for various yolk and "ghost" samples are sum- marized in Table II as nucleic acid phosphorus/nitrogen ratios. "Ghost" material gave the highest DNA-P/N () and PNA-P/N () Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration an
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