. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 S. W. JEFFREY AND F. T. HAXO (1953) for crystalline peridinin isolated from Prorocentrum micans were carried out. In all respects examined the properties of the two preparations were identical, , spectra before and after iodine catalysis of benzene solutions; number, adsorption position and spectral properties of isomers separable on CaCO3 columns; and percentage composition of an equilibrium mixture of stereo-isomers (57% all-trans and 43% combined cis-isomers). For comparison with one of the original sources used b
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 S. W. JEFFREY AND F. T. HAXO (1953) for crystalline peridinin isolated from Prorocentrum micans were carried out. In all respects examined the properties of the two preparations were identical, , spectra before and after iodine catalysis of benzene solutions; number, adsorption position and spectral properties of isomers separable on CaCO3 columns; and percentage composition of an equilibrium mixture of stereo-isomers (57% all-trans and 43% combined cis-isomers). For comparison with one of the original sources used by Strain et al. (1944), peridinin was isolated from zooxanthallae from the sea anemone Anthopleura xanthogramtnica. (Live sea anemones were collected intertidally at La Jolla.) Peridinins from the sea anemone and Tridacna gigas were found to be chromato- graphically homogeneous in the thin layer system, silica gel G-30% acetone in hexane. RESULTS Figure 1 shows a typical two-dimensional paper chromatogram of the pig- ments of zooxanthellae from Tridacna crocea. Identical chromatograms were THIN LAYER CHROMATOGRAM OF AMPHIDINIUM PIGMENTS ro O 9 08 07 06 2 05 O 4 03 O2 O-l O. O O-l O'2 O 3 O 4 O5 O6 O7 O 8 O9 I O O_ WAI MCC fc_ RF VALUES FIGURE 2. Sucrose thin layer chromatogram of pigments in the dinoflagellate Amphidinium sp. Chromatographic solvent systems; 1st dimension, n-propanol in petroleum ether (60-80°) ; 2nd dimension, 12% chloroform in petroleum ether. Pigment fractions as in Figure Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biolog
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
