. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Materials and Methods Arg 123456789 10 11 Sequencer Cycle b 100 i <u <J c o - o CtL 80 - 60 • 40 - 20 - 500 2500 Figure -I. Identification of lAMSFYFPRMamide from the most re- tained SCP-like immunoreactive peak (fractions 25-27) in Figure 2. a. The levels of pertinent amino acid derivatives at each cycle are plotted, and the amino acid assigned to each position is shown, b. FAB mass spectrum of this peak with the prominent molecular ion cused on the stimulation of feeding and gut motility (Murphy d a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Materials and Methods Arg 123456789 10 11 Sequencer Cycle b 100 i <u <J c o - o CtL 80 - 60 • 40 - 20 - 500 2500 Figure -I. Identification of lAMSFYFPRMamide from the most re- tained SCP-like immunoreactive peak (fractions 25-27) in Figure 2. a. The levels of pertinent amino acid derivatives at each cycle are plotted, and the amino acid assigned to each position is shown, b. FAB mass spectrum of this peak with the prominent molecular ion cused on the stimulation of feeding and gut motility (Murphy d at. 1985; Lloyd and Willows. 1988; Willows cl at. 1988; Prior and Welsford, 1989; and Krajniak a at. 1989). We sought to learn whether, and to what extent, the structure and functions of the SCPs are retained in the Bivalvia, a class of mollusks that has been diverging from the Gastropoda since the early Cambrian (Pojeta el at. 1973). We report here the isolation and sequencing of SCP analogs from the venerid clam. Mcrccnaria mercen- aria. from another heterodont, the giant Atlantic cockle Dinocardium robustrum, as well as from a pteriomorph, the eastern oyster Crassostrea virxinica. In M. mercenaiia. we have also determined the tissue distributions of the clam peptides and have demonstrated their actions on the musculature of several isolated organs. Preliminary reports of these data have been published (Price ct at. 1989; Can- delario el at. 1990a; Candelario el at. 1990b). Animals Quahogs (Mcrccnana mercenaria) and eastern oysters (Crassostrea virginica) were obtained from the inland waters adjacent to Marineland, Florida. Giant cockles (Dinocardium rohnsium) were obtained from the beach in the same locality. The bivalves were maintained in flowing, natural seawater until they were needed, except that only freshly collected animals were used for immu- nohistochemistry. Pcptifle characlcriialiiin Between 1 and 10 whole shucked animals, or batches of 10 or 20 pieces of partic
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
