. Ciba Foundation symposium on ionizing radiations and cell metabolism. Radiation -- Physiological effect; Cell metabolism; Radiation -- effects; Radioactivity. 216 Arne Forssberg case of RNA and proteins. Since the total activity of Land- schiitz ascites cells, labelled with [2-i4C]glycine, is diluted by cell multiplication more than could be expected from increase ill cell mass, Greenlees and LePage (1955), for instance, deduced an exchange (loss) of protein-bound activity amount- ing to about 9 per cent per day. Using the same material. O UJ q:/^100- UJ O CO o 5^ > 50- -J > -J — UJ H-
. Ciba Foundation symposium on ionizing radiations and cell metabolism. Radiation -- Physiological effect; Cell metabolism; Radiation -- effects; Radioactivity. 216 Arne Forssberg case of RNA and proteins. Since the total activity of Land- schiitz ascites cells, labelled with [2-i4C]glycine, is diluted by cell multiplication more than could be expected from increase ill cell mass, Greenlees and LePage (1955), for instance, deduced an exchange (loss) of protein-bound activity amount- ing to about 9 per cent per day. Using the same material. O UJ q:/^100- UJ O CO o 5^ > 50- -J > -J — UJ H- -:^ UJ U >Q 5 ^ UJ <? -J UJ Lk 1 oco '. -6 TUMOR CELL NUMBER- V- P DNA^ O SPECIFIC ACTIVITY 0F< RNA -o • • PROTEIN: A- 3 o -5? CO 1-4 = CO 4 5 6 DAYS AFTER INOCULATION Fig. 1. Total number of free tumour cells and inverted specific activity of DNA, RNA and proteins as a function of time after inoculation. All values were brought to a common scale (left) by taking the values corresponding to the inoculum as equal to one. Curves are drawn through the geometrical mean of the values from the different series, each value representing a pool of ascites from 4 to 15 mice. The right-hand scale shows the number of generations. Ledoux and Revell (1955) made the observation that the RNA concentration per cell decreased considerably with the age of the inoculated cell culture. Ehrlich tumour cells, with [8-i^C]adenine or [2-'^^C]g[ycme as precursors, were used in our studies on similar stability problems (Revesz, Forssberg and Klein, unpublished). In parallel analyses of specific activity and cell multiplication during the week following inoculation of the cells labelled in vivo, we obtained the results shown in Fig. 1. There is a. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenh
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