. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BIVALVE HEMOCYANIN 211. CcTMg* EDTA YOLDIA T T T 15S 96S AC I LA FIGURE 4. Scanner traces showing dissociation and reassociation of bivalve Hcs at pH , 20°C. Yoldia limanda: (1) in M Tris-HCl, 50 mM MgCL, 10 mM CaCl2; (2) dialyzed against A/Tris- HC1 + 10 mM EDTA; (3) dialyzed back again against the original buffer. Acila castrensis: (4) as in 1; (5) as in 2; (6) as in 3. In 5 and 6 the middle boundary sediments at about 55S. blood and then measuring the width of all (24) of the 12-tiered cylinders observed and
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BIVALVE HEMOCYANIN 211. CcTMg* EDTA YOLDIA T T T 15S 96S AC I LA FIGURE 4. Scanner traces showing dissociation and reassociation of bivalve Hcs at pH , 20°C. Yoldia limanda: (1) in M Tris-HCl, 50 mM MgCL, 10 mM CaCl2; (2) dialyzed against A/Tris- HC1 + 10 mM EDTA; (3) dialyzed back again against the original buffer. Acila castrensis: (4) as in 1; (5) as in 2; (6) as in 3. In 5 and 6 the middle boundary sediments at about 55S. blood and then measuring the width of all (24) of the 12-tiered cylinders observed and a sample of 100 6-tiered cylinders. The bivalve circles have a five-fold rotational symmetry and a collar and a cap. When dissociated to halves, the molecule looks like a three-tiered rectangle, which absorbs more stain at one end than the other, and as circles, only some of which have collars and caps (see Fig. 3 in Morse et ai, 1986). The images of half molecules, which have also been described for Helix He, are believed to reflect the absence of collars and caps at the broken surfaces (van Bruggen et ai, 1981). Like gastropod Hcs, the bivalve squares are about 35 nm long. Physical characterization Sedimentation velocity experiments with A. castrensis and Y. limatula Hcs were performed at room temperature and under a variety of solvent conditions. The results are summarized as follows: (1) In M Tris-HCl buffer (pH ) containing 50 mMCaCl2 and 10 mM MgCl2, both Hcs exhibited single, sharp boundaries. The sedimentation coefficients (S°2o,w), when corrected to standard conditions and extrapolated to zero He concen- tration, were for A. castrensis He and for Y. limatula He. (2) When the two He solutions were dialyzed exhaustively against 10 mMEDTA in M Tris-HCl (pH ), they behaved differently (Fig. 4). Y. limatula He disso- ciated completely to yield a single boundary with : Under the same conditions A. castrensis He showed incomplete di
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
