The Journal of biological chemistry . per gram as the proteins ofhorse serum (the slight difference in pH between plasma andfluid may be neglected). At this pH the formula (Equation 54)[BP] = [P] (pH - ) becomes [BP] = [P]. Thearterial HCO3 values are estimated by subtracting 2 mM. perliter from the values found in the venous serum. The estimated [Cl]s: [CI]/ ratios found coincide with the cal-culated Tsf values nearly within the limit of experimental error. Van Slyke, Wu, and McLean 793 The [HCOsJs : [HCO3]/ ratios are all higher than the calculated r^fwhen the venous values


The Journal of biological chemistry . per gram as the proteins ofhorse serum (the slight difference in pH between plasma andfluid may be neglected). At this pH the formula (Equation 54)[BP] = [P] (pH - ) becomes [BP] = [P]. Thearterial HCO3 values are estimated by subtracting 2 mM. perliter from the values found in the venous serum. The estimated [Cl]s: [CI]/ ratios found coincide with the cal-culated Tsf values nearly within the limit of experimental error. Van Slyke, Wu, and McLean 793 The [HCOsJs : [HCO3]/ ratios are all higher than the calculated r^fwhen the venous values for [HCOajs are used; but the estimatedarterial values for [HCOsjs yield [HCOsl^ • [HCO3]/ ratios whichagree with the calculated r^/ as closely as could be expected, whenthe possible magnitude of the error involved in assuming a con-stant difference between arterial and venous CO2, is considered. Total. => CO2 BHCO3 Per cent g Per centmn. mM. of Wood ^ of blood per kg. pepK^. rbloodiitilood H2CO3mn. pepkg, tilood CO 2tension Fig. 5. DOcagne-Henderson nomogram showing calculated relation-ships for arterial and venous blood of average serum and cell compositionobserved in our experiments. j The [Na]/ : [Na]s ratios agree, in six out of seven cases, with thecalculated r^/ values wdthin the rather wide limit of error assignedby the authors to the Na determination. The [K]/ : [K]s ratiosare altogether lower than the calculated Ts/, and are very source of the deviation and irregularity of the K ratios is at 794 Electrolyte and Water Distribution eO o e 0^s . o V.^ J ^ § <u -is ^-^ ft |< !^ oq a: S Jg -^ ta CO ^ =3 :§ Cq CO § ^^^ si ^•^^ O W CO lOCO -TfH o o «o 05 CO 3 O lO Id o o o «o lO l^ .-1 00 § 05 o C3 3 O 2 o CO o CO CO CO o ?* CO o o - o o OlCO-^OiOiOOOiMOO-n* OCOC5C3COiOOOo »o CO O O CO CO 05 CO lO 00 lO CO CO t


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